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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0277499 (DDB_G0277499) CSB-YP762337DKK
CSB-EP762337DKK
CSB-BP762337DKK
CSB-MP762337DKK
CSB-EP762337DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable serine/threonine-protein kinase DDB_G0277165 (DDB_G0277165), partial CSB-YP762338DKK
CSB-EP762338DKK
CSB-BP762338DKK
CSB-MP762338DKK
CSB-EP762338DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe LIM domain and RING finger protein C1223.01 (SPCC1223.01, SPCC285.18), partial CSB-YP762339SXV
CSB-EP762339SXV
CSB-BP762339SXV
CSB-MP762339SXV
CSB-EP762339SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 1 (M) CSB-YP762340IFR
CSB-EP762340IFR
CSB-BP762340IFR
CSB-MP762340IFR
CSB-EP762340IFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP762341IEP
CSB-EP762341IEP
CSB-BP762341IEP
CSB-MP762341IEP
CSB-EP762341IEP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 14.8 kDa protein in frd-Gp32 intergenic region (frd.2) CSB-YP762342BDL
CSB-EP762342BDL
CSB-BP762342BDL
CSB-MP762342BDL
CSB-EP762342BDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Matrix protein 1 (M) CSB-YP762343ICAG
CSB-EP762343ICAG
CSB-BP762343ICAG
CSB-MP762343ICAG
CSB-EP762343ICAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus DNA-directed RNA polymerase 133 kDa polypeptide (RPO132), partial CSB-YP762344VAI
CSB-EP762344VAI
CSB-BP762344VAI
CSB-MP762344VAI
CSB-EP762344VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Glutaredoxin-1 (MVA061L, ACAM3000_MVA_061) CSB-YP762345VEE
CSB-EP762345VEE
CSB-BP762345VEE
CSB-MP762345VEE
CSB-EP762345VEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group O Protein Nef (nef) CSB-YP762346HLS
CSB-EP762346HLS
CSB-BP762346HLS
CSB-MP762346HLS
CSB-EP762346HLS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Camelpox virus Intermediate transcription factor 3 large subunit (VITF3L) CSB-YP762347CBR
CSB-EP762347CBR
CSB-BP762347CBR
CSB-MP762347CBR
CSB-EP762347CBR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Camelpox virus Growth factor (CMP11R) CSB-YP762348CBR
CSB-EP762348CBR
CSB-BP762348CBR
CSB-MP762348CBR
CSB-EP762348CBR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zaire ebolavirus Matrix protein VP40 (VP40) CSB-YP762349ZAT
CSB-MP762349ZAT
CSB-EP762349ZAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Nucleoprotein (N), partial CSB-YP762350MJG
CSB-EP762350MJG
CSB-BP762350MJG
CSB-MP762350MJG
CSB-EP762350MJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Measles virus Nucleoprotein (N), partial CSB-YP762351MGAJ
CSB-EP762351MGAJ
CSB-BP762351MGAJ
CSB-MP762351MGAJ
CSB-EP762351MGAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gallid herpesvirus 2 Tegument protein UL55 homolog (MDV070) CSB-YP762352GAN
CSB-EP762352GAN
CSB-BP762352GAN
CSB-MP762352GAN
CSB-EP762352GAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gallid herpesvirus 2 Ribonucleoside-diphosphate reductase small chain (MDV053) CSB-YP762353GAN
CSB-EP762353GAN
CSB-BP762353GAN
CSB-MP762353GAN
CSB-EP762353GAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gallid herpesvirus 2 Envelope glycoprotein L (MDV013) CSB-YP762354GAN
CSB-EP762354GAN
CSB-BP762354GAN
CSB-MP762354GAN
CSB-EP762354GAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varicella-zoster virus Envelope glycoprotein I (gI), partial CSB-YP762355VAQ1
CSB-EP762355VAQ1
CSB-BP762355VAQ1
CSB-MP762355VAQ1
CSB-EP762355VAQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural glycoprotein 4, partial CSB-YP762356RGY
CSB-EP762356RGY
CSB-BP762356RGY
CSB-MP762356RGY
CSB-EP762356RGY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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