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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bartonella quintana UPF0102 protein BQ09720 (BQ09720) CSB-YP763801BSH
CSB-EP763801BSH
CSB-BP763801BSH
CSB-MP763801BSH
CSB-EP763801BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Transcription elongation factor GreA (greA) CSB-YP763802BSH
CSB-EP763802BSH
CSB-BP763802BSH
CSB-MP763802BSH
CSB-EP763802BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana tRNA dimethylallyltransferase (miaA) CSB-YP763803BSH
CSB-EP763803BSH
CSB-BP763803BSH
CSB-MP763803BSH
CSB-EP763803BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Elongation factor G (fusA), partial CSB-YP763804BSH
CSB-EP763804BSH
CSB-BP763804BSH
CSB-MP763804BSH
CSB-EP763804BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 30S ribosomal protein S19 (rpsS) CSB-YP763805BSH
CSB-EP763805BSH
CSB-BP763805BSH
CSB-MP763805BSH
CSB-EP763805BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP763806BSH
CSB-EP763806BSH
CSB-BP763806BSH
CSB-MP763806BSH
CSB-EP763806BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Phosphopantetheine adenylyltransferase (coaD) CSB-YP763807BSH
CSB-EP763807BSH
CSB-BP763807BSH
CSB-MP763807BSH
CSB-EP763807BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP763808BSH
CSB-EP763808BSH
CSB-BP763808BSH
CSB-MP763808BSH
CSB-EP763808BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP763809BSH
CSB-EP763809BSH
CSB-BP763809BSH
CSB-MP763809BSH
CSB-EP763809BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Glutamate racemase (murI) CSB-YP763810BSH
CSB-EP763810BSH
CSB-BP763810BSH
CSB-MP763810BSH
CSB-EP763810BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Tyrosine--tRNA ligase (tyrS) CSB-YP763811BSH
CSB-EP763811BSH
CSB-BP763811BSH
CSB-MP763811BSH
CSB-EP763811BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP763812BSH
CSB-EP763812BSH
CSB-BP763812BSH
CSB-MP763812BSH
CSB-EP763812BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Heme A synthase (ctaA), partial CSB-YP763813BSH
CSB-EP763813BSH
CSB-BP763813BSH
CSB-MP763813BSH
CSB-EP763813BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Dihydrodipicolinate synthase (dapA) CSB-YP763814BSH
CSB-EP763814BSH
CSB-BP763814BSH
CSB-MP763814BSH
CSB-EP763814BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP763815BSH
CSB-EP763815BSH
CSB-BP763815BSH
CSB-MP763815BSH
CSB-EP763815BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Ribonuclease HII (rnhB) CSB-YP763816BSH
CSB-EP763816BSH
CSB-BP763816BSH
CSB-MP763816BSH
CSB-EP763816BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana ATP synthase subunit b 2 (atpF2), partial CSB-YP763817BSH
CSB-EP763817BSH
CSB-BP763817BSH
CSB-MP763817BSH
CSB-EP763817BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana GTPase Der (der) CSB-YP763818BSH
CSB-EP763818BSH
CSB-BP763818BSH
CSB-MP763818BSH
CSB-EP763818BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP763819BSH
CSB-EP763819BSH
CSB-BP763819BSH
CSB-MP763819BSH
CSB-EP763819BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella quintana Protein translocase subunit SecA (secA), partial CSB-YP763820BSH
CSB-EP763820BSH
CSB-BP763820BSH
CSB-MP763820BSH
CSB-EP763820BSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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