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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Tyrosine--tRNA ligase (tyrS) CSB-YP763200SSL
CSB-EP763200SSL
CSB-BP763200SSL
CSB-MP763200SSL
CSB-EP763200SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 10 kDa chaperonin 2 (groS2) CSB-YP763201RDR
CSB-EP763201RDR
CSB-BP763201RDR
CSB-MP763201RDR
CSB-EP763201RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Glycine cleavage system H protein (gcvH) CSB-YP763202EYQ
CSB-EP763202EYQ
CSB-BP763202EYQ
CSB-MP763202EYQ
CSB-EP763202EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 30S ribosomal protein S20 (rpsT) CSB-YP763203EYQ
CSB-EP763203EYQ
CSB-BP763203EYQ
CSB-MP763203EYQ
CSB-EP763203EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris 30S ribosomal protein S16 (rpsP) CSB-YP763204EYQ
CSB-EP763204EYQ
CSB-BP763204EYQ
CSB-MP763204EYQ
CSB-EP763204EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP763205EYQ
CSB-EP763205EYQ
CSB-BP763205EYQ
CSB-MP763205EYQ
CSB-EP763205EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Acetylglutamate kinase (argB) CSB-YP763206EYQ
CSB-EP763206EYQ
CSB-BP763206EYQ
CSB-MP763206EYQ
CSB-EP763206EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris NAD (P)H-quinone oxidoreductase subunit I (ndhI) CSB-YP763207EYQ
CSB-EP763207EYQ
CSB-BP763207EYQ
CSB-MP763207EYQ
CSB-EP763207EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP763208EYO
CSB-EP763208EYO
CSB-BP763208EYO
CSB-MP763208EYO
CSB-EP763208EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus CinA-like protein (PMT_1845) CSB-YP763209EYO
CSB-EP763209EYO
CSB-BP763209EYO
CSB-MP763209EYO
CSB-EP763209EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein M (psbM), partial CSB-YP763210EYO
CSB-EP763210EYO
CSB-BP763210EYO
CSB-MP763210EYO
CSB-EP763210EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal protein L11 methyltransferase (prmA) CSB-YP763211EYO
CSB-EP763211EYO
CSB-BP763211EYO
CSB-MP763211EYO
CSB-EP763211EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 2-isopropylmalate synthase (leuA), partial CSB-YP763212EYO
CSB-EP763212EYO
CSB-BP763212EYO
CSB-MP763212EYO
CSB-EP763212EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Anthranilate phosphoribosyltransferase (trpD) CSB-YP763213EYO
CSB-EP763213EYO
CSB-BP763213EYO
CSB-MP763213EYO
CSB-EP763213EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP763214EYO
CSB-EP763214EYO
CSB-BP763214EYO
CSB-MP763214EYO
CSB-EP763214EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Translation initiation factor IF-3 (infC) CSB-YP763215EYO
CSB-EP763215EYO
CSB-BP763215EYO
CSB-MP763215EYO
CSB-EP763215EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 60 kDa chaperonin 2 (groL2), partial CSB-YP763216EYP
CSB-EP763216EYP
CSB-BP763216EYP
CSB-MP763216EYP
CSB-EP763216EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant FAD-containing monooxygenase EthA (ethA) CSB-YP763217MVH
CSB-EP763217MVH
CSB-BP763217MVH
CSB-MP763217MVH
CSB-EP763217MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable arabinosyltransferase C (embC), partial CSB-YP763218MVH
CSB-EP763218MVH
CSB-BP763218MVH
CSB-MP763218MVH
CSB-EP763218MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP763219MVH
CSB-EP763219MVH
CSB-BP763219MVH
CSB-MP763219MVH
CSB-EP763219MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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