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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Prochlorococcus marinus Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP763401EYO
CSB-EP763401EYO
CSB-BP763401EYO
CSB-MP763401EYO
CSB-EP763401EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Maf-like protein PMT_1181 (PMT_1181) CSB-YP763402EYO
CSB-EP763402EYO
CSB-BP763402EYO
CSB-MP763402EYO
CSB-EP763402EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Adaptive-response sensory-kinase sasA (sasA) CSB-YP763403EYO
CSB-EP763403EYO
CSB-BP763403EYO
CSB-MP763403EYO
CSB-EP763403EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Divinyl chlorophyll a/b light-harvesting protein pcbB (pcbB), partial CSB-YP763404EYO1
CSB-EP763404EYO1
CSB-BP763404EYO1
CSB-MP763404EYO1
CSB-EP763404EYO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Proton extrusion protein PcxA (pcxA), partial CSB-YP763405EYO1
CSB-EP763405EYO1
CSB-BP763405EYO1
CSB-MP763405EYO1
CSB-EP763405EYO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP763406EYO
CSB-EP763406EYO
CSB-BP763406EYO
CSB-MP763406EYO
CSB-EP763406EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b6-f complex subunit 8 (petN), partial CSB-YP763407EYO
CSB-EP763407EYO
CSB-BP763407EYO
CSB-MP763407EYO
CSB-EP763407EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP763408EYO
CSB-EP763408EYO
CSB-BP763408EYO
CSB-MP763408EYO
CSB-EP763408EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 3-isopropylmalate dehydrogenase (leuB) CSB-YP763409EYO
CSB-EP763409EYO
CSB-BP763409EYO
CSB-MP763409EYO
CSB-EP763409EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP763410EYO
CSB-EP763410EYO
CSB-BP763410EYO
CSB-MP763410EYO
CSB-EP763410EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable 2-phosphosulfolactate phosphatase (comB) CSB-YP763411EYO
CSB-EP763411EYO
CSB-BP763411EYO
CSB-MP763411EYO
CSB-EP763411EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Acetylornithine aminotransferase (argD) CSB-YP763412EYO
CSB-EP763412EYO
CSB-BP763412EYO
CSB-MP763412EYO
CSB-EP763412EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Elongation factor 4 (lepA), partial CSB-YP763413EYO
CSB-EP763413EYO
CSB-BP763413EYO
CSB-MP763413EYO
CSB-EP763413EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cell division protein SepF (sepF) CSB-YP763414EYO
CSB-EP763414EYO
CSB-BP763414EYO
CSB-MP763414EYO
CSB-EP763414EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP763415EYO
CSB-EP763415EYO
CSB-BP763415EYO
CSB-MP763415EYO
CSB-EP763415EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Protein CrcB homolog 1 (crcB1), partial CSB-YP763416EYO1
CSB-EP763416EYO1
CSB-BP763416EYO1
CSB-MP763416EYO1
CSB-EP763416EYO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus DNA mismatch repair protein MutS (mutS), partial CSB-YP763417EYO
CSB-EP763417EYO
CSB-BP763417EYO
CSB-MP763417EYO
CSB-EP763417EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Thiazole synthase (thiG) CSB-YP763418EYO
CSB-EP763418EYO
CSB-BP763418EYO
CSB-MP763418EYO
CSB-EP763418EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UPF0133 protein PMT_0025 (PMT_0025) CSB-YP763419EYO
CSB-EP763419EYO
CSB-BP763419EYO
CSB-MP763419EYO
CSB-EP763419EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Chaperone protein dnaK2 (dnaK2), partial CSB-YP763420EYP
CSB-EP763420EYP
CSB-BP763420EYP
CSB-MP763420EYP
CSB-EP763420EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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