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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP762478SMU
CSB-EP762478SMU
CSB-BP762478SMU
CSB-MP762478SMU
CSB-EP762478SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell cycle protein GpsB (gpsB) CSB-YP762479SMU
CSB-EP762479SMU
CSB-BP762479SMU
CSB-MP762479SMU
CSB-EP762479SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase gamma chain (atpG) CSB-YP762480SMU
CSB-EP762480SMU
CSB-BP762480SMU
CSB-MP762480SMU
CSB-EP762480SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L30 (rpmD) CSB-YP762481SMU
CSB-EP762481SMU
CSB-BP762481SMU
CSB-MP762481SMU
CSB-EP762481SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hypoxanthine-guanine phosphoribosyltransferase (hpt) CSB-YP762482SMU
CSB-EP762482SMU
CSB-BP762482SMU
CSB-MP762482SMU
CSB-EP762482SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Urease accessory protein UreG 1 (ureG1) CSB-YP762483BMO
CSB-EP762483BMO
CSB-BP762483BMO
CSB-MP762483BMO
CSB-EP762483BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium HTH-type transcriptional regulator UlaR (ulaR) CSB-YP762484SXB
CSB-EP762484SXB
CSB-BP762484SXB
CSB-MP762484SXB
CSB-EP762484SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium HTH-type transcriptional repressor fabR (fabR) CSB-YP762485SXB
CSB-EP762485SXB
CSB-BP762485SXB
CSB-MP762485SXB
CSB-EP762485SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium ATP synthase subunit b (atpF), partial CSB-YP762486SXB1
CSB-EP762486SXB1
CSB-BP762486SXB1
CSB-MP762486SXB1
CSB-EP762486SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium sn-glycerol-3-phosphate-binding periplasmic protein ugpB (ugpB) CSB-YP762487SXB
CSB-EP762487SXB
CSB-BP762487SXB
CSB-MP762487SXB
CSB-EP762487SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 50S ribosomal protein L21 (rplU) CSB-YP762488SXB
CSB-EP762488SXB
CSB-BP762488SXB
CSB-MP762488SXB
CSB-EP762488SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Bifunctional protein HldE (hldE) CSB-YP762489SXB
CSB-EP762489SXB
CSB-BP762489SXB
CSB-MP762489SXB
CSB-EP762489SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Protein grpE (grpE) CSB-YP762490SXB
CSB-EP762490SXB
CSB-BP762490SXB
CSB-MP762490SXB
CSB-EP762490SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP762491SXB
CSB-EP762491SXB
CSB-BP762491SXB
CSB-MP762491SXB
CSB-EP762491SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium tRNA (cmo5U34)-methyltransferase (cmoA) CSB-YP762492SXB
CSB-EP762492SXB
CSB-BP762492SXB
CSB-MP762492SXB
CSB-EP762492SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Spermidine export protein MdtI (mdtI), partial CSB-YP762493SXB1
CSB-EP762493SXB1
CSB-BP762493SXB1
CSB-MP762493SXB1
CSB-EP762493SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Leucine efflux protein (leuE), partial CSB-YP762494SXB1
CSB-EP762494SXB1
CSB-BP762494SXB1
CSB-MP762494SXB1
CSB-EP762494SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Allantoinase (allB) CSB-YP762495SXB
CSB-EP762495SXB
CSB-BP762495SXB
CSB-MP762495SXB
CSB-EP762495SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Cell division protein FtsQ (ftsQ), partial CSB-YP762496SXB
CSB-EP762496SXB
CSB-BP762496SXB
CSB-MP762496SXB
CSB-EP762496SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Thr operon leader peptide (thrL) CSB-YP762497SXB
CSB-EP762497SXB
CSB-BP762497SXB
CSB-MP762497SXB
CSB-EP762497SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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