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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio vulnificus Na (+)-translocating NADH-quinone reductase subunit F (nqrF), partial CSB-YP762678VCQ
CSB-EP762678VCQ
CSB-BP762678VCQ
CSB-MP762678VCQ
CSB-EP762678VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0325 protein VV2560 (VV2560) CSB-YP762679VCQ
CSB-EP762679VCQ
CSB-BP762679VCQ
CSB-MP762679VCQ
CSB-EP762679VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP762680VCQ
CSB-EP762680VCQ
CSB-BP762680VCQ
CSB-MP762680VCQ
CSB-EP762680VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribonuclease H (rnhA) CSB-YP762681VCQ
CSB-EP762681VCQ
CSB-BP762681VCQ
CSB-MP762681VCQ
CSB-EP762681VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP762682VCQ
CSB-EP762682VCQ
CSB-BP762682VCQ
CSB-MP762682VCQ
CSB-EP762682VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0133 protein VV2410 (VV2410) CSB-YP762683VCQ
CSB-EP762683VCQ
CSB-BP762683VCQ
CSB-MP762683VCQ
CSB-EP762683VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0227 protein VV2369 (VV2369) CSB-YP762684VCQ
CSB-EP762684VCQ
CSB-BP762684VCQ
CSB-MP762684VCQ
CSB-EP762684VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP762685VCQ
CSB-EP762685VCQ
CSB-BP762685VCQ
CSB-MP762685VCQ
CSB-EP762685VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Chromosome partition protein MukB (mukB), partial CSB-YP762686VCQ
CSB-EP762686VCQ
CSB-BP762686VCQ
CSB-MP762686VCQ
CSB-EP762686VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Quinolinate synthase A (nadA) CSB-YP762687VCQ
CSB-EP762687VCQ
CSB-BP762687VCQ
CSB-MP762687VCQ
CSB-EP762687VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0283 membrane protein VV2076 (VV2076), partial CSB-YP762688VCQ1
CSB-EP762688VCQ1
CSB-BP762688VCQ1
CSB-MP762688VCQ1
CSB-EP762688VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP762689VCQ
CSB-EP762689VCQ
CSB-BP762689VCQ
CSB-MP762689VCQ
CSB-EP762689VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus N-acetyl-D-glucosamine kinase (nagK) CSB-YP762690VCQ
CSB-EP762690VCQ
CSB-BP762690VCQ
CSB-MP762690VCQ
CSB-EP762690VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Macrodomain Ter protein (matP) CSB-YP762691VCQ
CSB-EP762691VCQ
CSB-BP762691VCQ
CSB-MP762691VCQ
CSB-EP762691VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP762692VCQ
CSB-EP762692VCQ
CSB-BP762692VCQ
CSB-MP762692VCQ
CSB-EP762692VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Cobalamin synthase (cobS), partial CSB-YP762693VCQ1
CSB-EP762693VCQ1
CSB-BP762693VCQ1
CSB-MP762693VCQ1
CSB-EP762693VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Putative reductase VV1437 (VV1437) CSB-YP762694VCQ
CSB-EP762694VCQ
CSB-BP762694VCQ
CSB-MP762694VCQ
CSB-EP762694VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Cysteine--tRNA ligase (cysS) CSB-YP762695VCQ
CSB-EP762695VCQ
CSB-BP762695VCQ
CSB-MP762695VCQ
CSB-EP762695VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Histidinol-phosphate aminotransferase (hisC) CSB-YP762696VCQ
CSB-EP762696VCQ
CSB-BP762696VCQ
CSB-MP762696VCQ
CSB-EP762696VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Biotin synthase (bioB) CSB-YP762697VCQ
CSB-EP762697VCQ
CSB-BP762697VCQ
CSB-MP762697VCQ
CSB-EP762697VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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