Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bordetella bronchiseptica ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP763741BTY
CSB-EP763741BTY
CSB-BP763741BTY
CSB-MP763741BTY
CSB-EP763741BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Translation initiation factor IF-3 (infC) CSB-YP763742BTY
CSB-EP763742BTY
CSB-BP763742BTY
CSB-MP763742BTY
CSB-EP763742BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP763743BTY
CSB-EP763743BTY
CSB-BP763743BTY
CSB-MP763743BTY
CSB-EP763743BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP763744BTY
CSB-EP763744BTY
CSB-BP763744BTY
CSB-MP763744BTY
CSB-EP763744BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Probable potassium transport system protein kup (kup), partial CSB-YP763745BTY
CSB-EP763745BTY
CSB-BP763745BTY
CSB-MP763745BTY
CSB-EP763745BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP763746BTY
CSB-EP763746BTY
CSB-BP763746BTY
CSB-MP763746BTY
CSB-EP763746BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP763747BTY
CSB-EP763747BTY
CSB-BP763747BTY
CSB-MP763747BTY
CSB-EP763747BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP763748BTY
CSB-EP763748BTY
CSB-BP763748BTY
CSB-MP763748BTY
CSB-EP763748BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Uncharacterized transporter BB1538 (BB1538), partial CSB-YP763749BTY
CSB-EP763749BTY
CSB-BP763749BTY
CSB-MP763749BTY
CSB-EP763749BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Translation initiation factor IF-1 2 (infA2) CSB-YP763750BTY
CSB-EP763750BTY
CSB-BP763750BTY
CSB-MP763750BTY
CSB-EP763750BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Biotin synthase (bioB) CSB-YP763751BTY
CSB-EP763751BTY
CSB-BP763751BTY
CSB-MP763751BTY
CSB-EP763751BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Uncharacterized ribulose bisphosphate carboxylase-like protein (BB1035) CSB-YP763752BTY
CSB-EP763752BTY
CSB-BP763752BTY
CSB-MP763752BTY
CSB-EP763752BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Phosphopantetheine adenylyltransferase (coaD) CSB-YP763753BTY
CSB-EP763753BTY
CSB-BP763753BTY
CSB-MP763753BTY
CSB-EP763753BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP763754BTY
CSB-EP763754BTY
CSB-BP763754BTY
CSB-MP763754BTY
CSB-EP763754BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Molybdenum import ATP-binding protein ModC (modC) CSB-YP763755BTY
CSB-EP763755BTY
CSB-BP763755BTY
CSB-MP763755BTY
CSB-EP763755BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 7-cyano-7-deazaguanine synthase (queC) CSB-YP763756BTY
CSB-EP763756BTY
CSB-BP763756BTY
CSB-MP763756BTY
CSB-EP763756BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica Phosphomethylpyrimidine synthase (thiC), partial CSB-YP763757BTY
CSB-EP763757BTY
CSB-BP763757BTY
CSB-MP763757BTY
CSB-EP763757BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica S-adenosylmethionine synthase (metK) CSB-YP763758BTY
CSB-EP763758BTY
CSB-BP763758BTY
CSB-MP763758BTY
CSB-EP763758BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 3-dehydroquinate synthase (aroB) CSB-YP763759BTY
CSB-EP763759BTY
CSB-BP763759BTY
CSB-MP763759BTY
CSB-EP763759BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica 30S ribosomal protein S5 (rpsE) CSB-YP763760BTY
CSB-EP763760BTY
CSB-BP763760BTY
CSB-MP763760BTY
CSB-EP763760BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>