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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Gloeobacter violaceus Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP762878GCI
CSB-EP762878GCI
CSB-BP762878GCI
CSB-MP762878GCI
CSB-EP762878GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Lysine--tRNA ligase (lysS) CSB-YP762879GCI
CSB-EP762879GCI
CSB-BP762879GCI
CSB-MP762879GCI
CSB-EP762879GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP762880GCI
CSB-EP762880GCI
CSB-BP762880GCI
CSB-MP762880GCI
CSB-EP762880GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP762881GCI
CSB-EP762881GCI
CSB-BP762881GCI
CSB-MP762881GCI
CSB-EP762881GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP762882GCI
CSB-EP762882GCI
CSB-BP762882GCI
CSB-MP762882GCI
CSB-EP762882GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus 50S ribosomal protein L20 (rplT) CSB-YP762883GCI
CSB-EP762883GCI
CSB-BP762883GCI
CSB-MP762883GCI
CSB-EP762883GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Translation initiation factor IF-2 (infB), partial CSB-YP762884GCI
CSB-EP762884GCI
CSB-BP762884GCI
CSB-MP762884GCI
CSB-EP762884GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Phycocyanobilin:ferredoxin oxidoreductase (pcyA) CSB-YP762885GCI
CSB-EP762885GCI
CSB-BP762885GCI
CSB-MP762885GCI
CSB-EP762885GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus 3-dehydroquinate synthase (aroB) CSB-YP762886GCI
CSB-EP762886GCI
CSB-BP762886GCI
CSB-MP762886GCI
CSB-EP762886GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus 3-dehydroquinate dehydratase (aroQ) CSB-YP762887GCI
CSB-EP762887GCI
CSB-BP762887GCI
CSB-MP762887GCI
CSB-EP762887GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus S-methyl-5'-thioadenosine phosphorylase (mtnP) CSB-YP762888GCI
CSB-EP762888GCI
CSB-BP762888GCI
CSB-MP762888GCI
CSB-EP762888GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus NAD (P)H-quinone oxidoreductase subunit H 1 (ndhH1) CSB-YP762889GCI
CSB-EP762889GCI
CSB-BP762889GCI
CSB-MP762889GCI
CSB-EP762889GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Phosphoglycerate kinase (pgk) CSB-YP762890GCI
CSB-EP762890GCI
CSB-BP762890GCI
CSB-MP762890GCI
CSB-EP762890GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Photosystem I reaction center subunit XI (psaL), partial CSB-YP762891GCI1
CSB-EP762891GCI1
CSB-BP762891GCI1
CSB-MP762891GCI1
CSB-EP762891GCI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP762892GCI
CSB-EP762892GCI
CSB-BP762892GCI
CSB-MP762892GCI
CSB-EP762892GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Cell division topological specificity factor (minE) CSB-YP762893GCI
CSB-EP762893GCI
CSB-BP762893GCI
CSB-MP762893GCI
CSB-EP762893GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus UDP-3-O-acylglucosamine N-acyltransferase 1 (lpxD1) CSB-YP762894GCI
CSB-EP762894GCI
CSB-BP762894GCI
CSB-MP762894GCI
CSB-EP762894GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Cobyric acid synthase (cobQ) CSB-YP762895GCI
CSB-EP762895GCI
CSB-BP762895GCI
CSB-MP762895GCI
CSB-EP762895GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Sugar fermentation stimulation protein homolog (sfsA) CSB-YP762896GCI
CSB-EP762896GCI
CSB-BP762896GCI
CSB-MP762896GCI
CSB-EP762896GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloeobacter violaceus Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP762897GCI
CSB-EP762897GCI
CSB-BP762897GCI
CSB-MP762897GCI
CSB-EP762897GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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