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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis DIS3-like exonuclease 1 (dis3l), partial CSB-YP764101XBE
CSB-EP764101XBE
CSB-BP764101XBE
CSB-MP764101XBE
CSB-EP764101XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Proteasomal ubiquitin receptor ADRM1-A (adrm1-a) CSB-YP764102XBE
CSB-EP764102XBE
CSB-BP764102XBE
CSB-MP764102XBE
CSB-EP764102XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase rnf152-A (rnf152-a), partial CSB-YP764103XBE1
CSB-EP764103XBE1
CSB-BP764103XBE1
CSB-MP764103XBE1
CSB-EP764103XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein BCCIP homolog (bccip) CSB-YP764104XBE
CSB-EP764104XBE
CSB-BP764104XBE
CSB-MP764104XBE
CSB-EP764104XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein FAM102A (fam102a) CSB-YP764105XBE
CSB-EP764105XBE
CSB-BP764105XBE
CSB-MP764105XBE
CSB-EP764105XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis ATPase asna1 (asna1) CSB-YP764106XBE
CSB-EP764106XBE
CSB-BP764106XBE
CSB-MP764106XBE
CSB-EP764106XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0454 protein C12orf49 homolog CSB-YP764107XBE
CSB-EP764107XBE
CSB-BP764107XBE
CSB-MP764107XBE
CSB-EP764107XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Formin-binding protein 1 homolog (fnbp1), partial CSB-YP764108XBE
CSB-EP764108XBE
CSB-BP764108XBE
CSB-MP764108XBE
CSB-EP764108XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Oligosaccharyltransferase complex subunit ostc-A (ostc-a), partial CSB-YP764109XBE1
CSB-EP764109XBE1
CSB-BP764109XBE1
CSB-MP764109XBE1
CSB-EP764109XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nucleolar protein 16 (nop16) CSB-YP764110XBE
CSB-EP764110XBE
CSB-BP764110XBE
CSB-MP764110XBE
CSB-EP764110XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human XIAP-associated factor 1 (XAF1) CSB-YP764111HU
CSB-EP764111HU
CSB-BP764111HU
CSB-MP764111HU
CSB-EP764111HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis BRCA1-A complex subunit BRE (bre) CSB-YP764112XBE
CSB-EP764112XBE
CSB-BP764112XBE
CSB-MP764112XBE
CSB-EP764112XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Major facilitator superfamily domain-containing protein 8 (mfsd8), partial CSB-YP764113XBE
CSB-EP764113XBE
CSB-BP764113XBE
CSB-MP764113XBE
CSB-EP764113XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis CDC42 small effector protein 1-B (cdc42se1-b) CSB-YP764114XBE
CSB-EP764114XBE
CSB-BP764114XBE
CSB-MP764114XBE
CSB-EP764114XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Uncharacterized glycosyltransferase AER61 (aer61), partial CSB-YP764115XBE
CSB-EP764115XBE
CSB-BP764115XBE
CSB-MP764115XBE
CSB-EP764115XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Membrane magnesium transporter 1-A (mmgt1-a), partial CSB-YP764116XBE
CSB-EP764116XBE
CSB-BP764116XBE
CSB-MP764116XBE
CSB-EP764116XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis FAST kinase domain-containing protein 1 (fastkd1), partial CSB-YP764117XBE
CSB-EP764117XBE
CSB-BP764117XBE
CSB-MP764117XBE
CSB-EP764117XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Kinetochore protein Nuf2-B (nuf2-b) CSB-YP764118XBE
CSB-EP764118XBE
CSB-BP764118XBE
CSB-MP764118XBE
CSB-EP764118XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mediator of RNA polymerase II transcription subunit 22 (med22) CSB-YP764119XBE
CSB-EP764119XBE
CSB-BP764119XBE
CSB-MP764119XBE
CSB-EP764119XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein mab-21-like 1 (mab21l1) CSB-YP764120XBE
CSB-EP764120XBE
CSB-BP764120XBE
CSB-MP764120XBE
CSB-EP764120XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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