Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio tRNA-splicing ligase RtcB homolog (zgc:76871), partial CSB-YP764701DIL
CSB-EP764701DIL
CSB-BP764701DIL
CSB-MP764701DIL
CSB-EP764701DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Interferon regulatory factor 2-binding protein 2-A (irf2bp2a) CSB-YP764702DIL
CSB-EP764702DIL
CSB-BP764702DIL
CSB-MP764702DIL
CSB-EP764702DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C8orf74 (C8orf74) CSB-YP764703HU
CSB-EP764703HU
CSB-BP764703HU
CSB-MP764703HU
CSB-EP764703HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Lipoma HMGIC fusion partner-like 2 protein (lhfpl2), partial CSB-YP764704DIL1
CSB-EP764704DIL1
CSB-BP764704DIL1
CSB-MP764704DIL1
CSB-EP764704DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Probable cytosolic iron-sulfur protein assembly protein ciao1 (ciao1) CSB-YP764705DIL
CSB-EP764705DIL
CSB-BP764705DIL
CSB-MP764705DIL
CSB-EP764705DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Carbonyl reductase family member 4 (cbr4) CSB-YP764706DIL
CSB-EP764706DIL
CSB-BP764706DIL
CSB-MP764706DIL
CSB-EP764706DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Putative N-acetylglucosamine-6-phosphate deacetylase (amdhd2) CSB-YP764707DIL
CSB-EP764707DIL
CSB-BP764707DIL
CSB-MP764707DIL
CSB-EP764707DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Hyccin (fam126a), partial CSB-YP764708DIL
CSB-EP764708DIL
CSB-BP764708DIL
CSB-MP764708DIL
CSB-EP764708DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Inositol-pentakisphosphate 2-kinase (Ippk) CSB-YP764709MO
CSB-EP764709MO
CSB-BP764709MO
CSB-MP764709MO
CSB-EP764709MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Coiled-coil domain-containing protein 111 (Ccdc111) CSB-YP764710MO
CSB-EP764710MO
CSB-BP764710MO
CSB-MP764710MO
CSB-EP764710MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Lethal (2) giant larvae protein homolog 2 (LLGL2), partial CSB-YP764711HU
CSB-EP764711HU
CSB-BP764711HU
CSB-MP764711HU
CSB-EP764711HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human tRNA-dihydrouridine (16/17) synthase [NAD (P) (+)]-like (DUS1L) CSB-YP764712HU
CSB-EP764712HU
CSB-BP764712HU
CSB-MP764712HU
CSB-EP764712HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Tudor domain-containing protein 3 (tdrd3), partial CSB-YP764713XBF
CSB-EP764713XBF
CSB-BP764713XBF
CSB-MP764713XBF
CSB-EP764713XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Denticleless protein homolog (dtl), partial CSB-YP764714XBF
CSB-EP764714XBF
CSB-BP764714XBF
CSB-MP764714XBF
CSB-EP764714XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis E3 ubiquitin-protein ligase CHFR (chfr), partial CSB-YP764715XBF
CSB-EP764715XBF
CSB-BP764715XBF
CSB-MP764715XBF
CSB-EP764715XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mediator of RNA polymerase II transcription subunit 27 (MED27) CSB-YP764716HU
CSB-EP764716HU
CSB-BP764716HU
CSB-MP764716HU
CSB-EP764716HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Endogenous Bornavirus-like nucleoprotein 2 (EBLN2) CSB-YP764717HU
CSB-EP764717HU
CSB-BP764717HU
CSB-MP764717HU
CSB-EP764717HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative protein arginine N-methyltransferase 10 (PRMT10), partial CSB-YP764718HU
CSB-EP764718HU
CSB-BP764718HU
CSB-MP764718HU
CSB-EP764718HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Monocarboxylate transporter 12 (slc16a12), partial CSB-YP764719XBF
CSB-EP764719XBF
CSB-BP764719XBF
CSB-MP764719XBF
CSB-EP764719XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Mitochondrial import inner membrane translocase subunit Tim10 (timm10) CSB-YP764720XBF
CSB-EP764720XBF
CSB-BP764720XBF
CSB-MP764720XBF
CSB-EP764720XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>