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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Ribosome-recycling factor (frr) CSB-YP764181BAAE
CSB-EP764181BAAE
CSB-BP764181BAAE
CSB-MP764181BAAE
CSB-EP764181BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S15 (rpsO) CSB-YP764182BAAE
CSB-EP764182BAAE
CSB-BP764182BAAE
CSB-MP764182BAAE
CSB-EP764182BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0291 protein BT9727_3443 (BT9727_3443) CSB-YP764183BAAE
CSB-EP764183BAAE
CSB-BP764183BAAE
CSB-MP764183BAAE
CSB-EP764183BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Hut operon positive regulatory protein (hutP) CSB-YP764184BAAE
CSB-EP764184BAAE
CSB-BP764184BAAE
CSB-MP764184BAAE
CSB-EP764184BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0061 protein BT9727_3274 (BT9727_3274) CSB-YP764185BAAE
CSB-EP764185BAAE
CSB-BP764185BAAE
CSB-MP764185BAAE
CSB-EP764185BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 1-deoxy-D-xylulose 5-phosphate reductoisomerase 1 (dxr1) CSB-YP764186BAAE
CSB-EP764186BAAE
CSB-BP764186BAAE
CSB-MP764186BAAE
CSB-EP764186BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Endonuclease V (nfi) CSB-YP764187BAAE
CSB-EP764187BAAE
CSB-BP764187BAAE
CSB-MP764187BAAE
CSB-EP764187BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian NAD-dependent protein deacetylase (cobB) CSB-YP764188BAAE
CSB-EP764188BAAE
CSB-BP764188BAAE
CSB-MP764188BAAE
CSB-EP764188BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Chorismate synthase 2 (aroC2) CSB-YP764189BAAE
CSB-EP764189BAAE
CSB-BP764189BAAE
CSB-MP764189BAAE
CSB-EP764189BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP764190BAAE
CSB-EP764190BAAE
CSB-BP764190BAAE
CSB-MP764190BAAE
CSB-EP764190BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Methylmalonate semialdehyde dehydrogenase [acylating] 2 (iolA2) CSB-YP764191BAAE
CSB-EP764191BAAE
CSB-BP764191BAAE
CSB-MP764191BAAE
CSB-EP764191BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Isoleucine--tRNA ligase 2 (ileS2), partial CSB-YP764192BAAE
CSB-EP764192BAAE
CSB-BP764192BAAE
CSB-MP764192BAAE
CSB-EP764192BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian FMN-dependent NADH-azoreductase 1 (azoR1) CSB-YP764193BAAE
CSB-EP764193BAAE
CSB-BP764193BAAE
CSB-MP764193BAAE
CSB-EP764193BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Holliday junction resolvase recU (recU) CSB-YP764194BAAE
CSB-EP764194BAAE
CSB-BP764194BAAE
CSB-MP764194BAAE
CSB-EP764194BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Chorismate synthase 1 (aroC1) CSB-YP764195BAAE
CSB-EP764195BAAE
CSB-BP764195BAAE
CSB-MP764195BAAE
CSB-EP764195BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Sulfate adenylyltransferase (sat) CSB-YP764196BAAE
CSB-EP764196BAAE
CSB-BP764196BAAE
CSB-MP764196BAAE
CSB-EP764196BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ketol-acid reductoisomerase 1 (ilvC1) CSB-YP764197BAAE
CSB-EP764197BAAE
CSB-BP764197BAAE
CSB-MP764197BAAE
CSB-EP764197BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 7-cyano-7-deazaguanine synthase (queC) CSB-YP764198BAAE
CSB-EP764198BAAE
CSB-BP764198BAAE
CSB-MP764198BAAE
CSB-EP764198BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0477 protein BT9727_1129 (BT9727_1129) CSB-YP764199BAAE
CSB-EP764199BAAE
CSB-BP764199BAAE
CSB-MP764199BAAE
CSB-EP764199BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0234 protein BT9727_1064 (BT9727_1064) CSB-YP764200BAAE
CSB-EP764200BAAE
CSB-BP764200BAAE
CSB-MP764200BAAE
CSB-EP764200BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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