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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Metastasis-suppressor KiSS-1 (Kiss1) CSB-YP765002MO
CSB-EP765002MO
CSB-BP765002MO
CSB-MP765002MO
CSB-EP765002MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma hominis 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) CSB-YP765003MWJ
CSB-EP765003MWJ
CSB-BP765003MWJ
CSB-MP765003MWJ
CSB-EP765003MWJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Anaphase-promoting complex subunit CDC26 (Cdc26) CSB-YP765004RA
CSB-EP765004RA
CSB-BP765004RA
CSB-MP765004RA
CSB-EP765004RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dengue virus type 3 Genome polyprotein (pol), partial CSB-YP765005DCAT
CSB-EP765005DCAT
CSB-BP765005DCAT
CSB-MP765005DCAT
CSB-EP765005DCAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botrytis virus X Uncharacterized ORF5 protein (ORF5) CSB-YP765006BDAR
CSB-EP765006BDAR
CSB-BP765006BDAR
CSB-MP765006BDAR
CSB-EP765006BDAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botrytis virus X Uncharacterized ORF2 protein (ORF2) CSB-YP765007BDAR
CSB-EP765007BDAR
CSB-BP765007BDAR
CSB-MP765007BDAR
CSB-EP765007BDAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative nitric oxide synthase (Os02g0104700, LOC_Os02g01440), partial CSB-YP765008OFG
CSB-EP765008OFG
CSB-BP765008OFG
CSB-MP765008OFG
CSB-EP765008OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Putative septation protein spoVG (spoVG) CSB-YP765009OBN
CSB-EP765009OBN
CSB-BP765009OBN
CSB-MP765009OBN
CSB-EP765009OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Probable rRNA maturation factor (PAM_624) CSB-YP765010OBN
CSB-EP765010OBN
CSB-BP765010OBN
CSB-MP765010OBN
CSB-EP765010OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Lysine--tRNA ligase (lysS), partial CSB-YP765011OBN
CSB-EP765011OBN
CSB-BP765011OBN
CSB-MP765011OBN
CSB-EP765011OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Thymidylate synthase (thyA) CSB-YP765012OBN
CSB-EP765012OBN
CSB-BP765012OBN
CSB-MP765012OBN
CSB-EP765012OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP765013OBN
CSB-EP765013OBN
CSB-BP765013OBN
CSB-MP765013OBN
CSB-EP765013OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Cytidylate kinase (cmk) CSB-YP765014OBN
CSB-EP765014OBN
CSB-BP765014OBN
CSB-MP765014OBN
CSB-EP765014OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Adenylate kinase (adk) CSB-YP765015OBN
CSB-EP765015OBN
CSB-BP765015OBN
CSB-MP765015OBN
CSB-EP765015OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 30S ribosomal protein S3 (rpsC) CSB-YP765016OBN
CSB-EP765016OBN
CSB-BP765016OBN
CSB-MP765016OBN
CSB-EP765016OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L23 (rplW) CSB-YP765017OBN
CSB-EP765017OBN
CSB-BP765017OBN
CSB-MP765017OBN
CSB-EP765017OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Energy-coupling factor transporter ATP-binding protein EcfA (ecfA) CSB-YP765018OBN
CSB-EP765018OBN
CSB-BP765018OBN
CSB-MP765018OBN
CSB-EP765018OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma tRNA (Ile)-lysidine synthase (tilS) CSB-YP765019OBN
CSB-EP765019OBN
CSB-BP765019OBN
CSB-MP765019OBN
CSB-EP765019OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 60 kDa chaperonin (groL), partial CSB-YP765020OBN
CSB-EP765020OBN
CSB-BP765020OBN
CSB-MP765020OBN
CSB-EP765020OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma 50S ribosomal protein L27 (rpmA) CSB-YP765021OBN
CSB-EP765021OBN
CSB-BP765021OBN
CSB-MP765021OBN
CSB-EP765021OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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