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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rat Hermansky-Pudlak syndrome 6 protein homolog (Hps6), partial CSB-YP766002RA
CSB-EP766002RA
CSB-BP766002RA
CSB-MP766002RA
CSB-EP766002RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse N-acetylated-alpha-linked acidic dipeptidase-like protein (Naaladl1), partial CSB-YP766003MO
CSB-EP766003MO
CSB-BP766003MO
CSB-MP766003MO
CSB-EP766003MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Elongation factor Tu (tuf1) CSB-YP766004CKA
CSB-EP766004CKA
CSB-BP766004CKA
CSB-MP766004CKA
CSB-EP766004CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 60 kDa chaperonin 1 (groL1), partial CSB-YP766005VCQ
CSB-EP766005VCQ
CSB-BP766005VCQ
CSB-MP766005VCQ
CSB-EP766005VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP766006WBQ
CSB-EP766006WBQ
CSB-BP766006WBQ
CSB-MP766006WBQ
CSB-EP766006WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dephospho-CoA kinase (coaE) CSB-YP766007WBQ
CSB-EP766007WBQ
CSB-BP766007WBQ
CSB-MP766007WBQ
CSB-EP766007WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (uracil (54)-C (5))-methyltransferase (trmA) CSB-YP766008WBQ
CSB-EP766008WBQ
CSB-BP766008WBQ
CSB-MP766008WBQ
CSB-EP766008WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) CSB-YP766009WBQ
CSB-EP766009WBQ
CSB-BP766009WBQ
CSB-MP766009WBQ
CSB-EP766009WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP766010WBQ
CSB-EP766010WBQ
CSB-BP766010WBQ
CSB-MP766010WBQ
CSB-EP766010WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphomethylpyrimidine synthase (thiC) CSB-YP766011WBQ
CSB-EP766011WBQ
CSB-BP766011WBQ
CSB-MP766011WBQ
CSB-EP766011WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP766012WBQ
CSB-EP766012WBQ
CSB-BP766012WBQ
CSB-MP766012WBQ
CSB-EP766012WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L4 (rplD) CSB-YP766013WBQ
CSB-EP766013WBQ
CSB-BP766013WBQ
CSB-MP766013WBQ
CSB-EP766013WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14 (rplN) CSB-YP766014WBQ
CSB-EP766014WBQ
CSB-BP766014WBQ
CSB-MP766014WBQ
CSB-EP766014WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-1 (infA) CSB-YP766015WBQ
CSB-EP766015WBQ
CSB-BP766015WBQ
CSB-MP766015WBQ
CSB-EP766015WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP766016WBQ
CSB-EP766016WBQ
CSB-BP766016WBQ
CSB-MP766016WBQ
CSB-EP766016WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP766017WBQ
CSB-EP766017WBQ
CSB-BP766017WBQ
CSB-MP766017WBQ
CSB-EP766017WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrolide export ATP-binding/permease protein MacB (macB), partial CSB-YP766018WBQ1
CSB-EP766018WBQ1
CSB-BP766018WBQ1
CSB-MP766018WBQ1
CSB-EP766018WBQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP766019WBQ
CSB-EP766019WBQ
CSB-BP766019WBQ
CSB-MP766019WBQ
CSB-EP766019WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase (uppP), partial CSB-YP766020WBQ1
CSB-EP766020WBQ1
CSB-BP766020WBQ1
CSB-MP766020WBQ1
CSB-EP766020WBQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malate dehydrogenase (mdh) CSB-YP766021WBQ
CSB-EP766021WBQ
CSB-BP766021WBQ
CSB-MP766021WBQ
CSB-EP766021WBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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