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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dictyostelium discoideum Phosducin-like protein 1 (phlp1) CSB-YP765142DKK
CSB-EP765142DKK
CSB-BP765142DKK
CSB-MP765142DKK
CSB-EP765142DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat E3 ubiquitin-protein ligase SH3RF1 (Sh3rf1), partial CSB-YP765143RA
CSB-EP765143RA
CSB-BP765143RA
CSB-MP765143RA
CSB-EP765143RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viverra zibetha Cytochrome b (MT-CYB), partial CSB-YP765144VAAZ
CSB-EP765144VAAZ
CSB-BP765144VAAZ
CSB-MP765144VAAZ
CSB-EP765144VAAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spirogyra maxima Cytochrome b6 (petB), partial CSB-YP765145FKM1
CSB-EP765145FKM1
CSB-BP765145FKM1
CSB-MP765145FKM1
CSB-EP765145FKM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stangeria eriopus Cytochrome b559 subunit beta (psbF), partial CSB-YP765146SDAC
CSB-EP765146SDAC
CSB-BP765146SDAC
CSB-MP765146SDAC
CSB-EP765146SDAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bowenia serrulata Photosystem II reaction center protein L (psbL), partial CSB-YP765147BDAM
CSB-EP765147BDAM
CSB-BP765147BDAM
CSB-MP765147BDAM
CSB-EP765147BDAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein psbN (psbN), partial CSB-YP765148MES
CSB-EP765148MES
CSB-BP765148MES
CSB-MP765148MES
CSB-EP765148MES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Histone H3.3 (H3F3A) CSB-YP765149PI
CSB-EP765149PI
CSB-BP765149PI
CSB-MP765149PI
CSB-EP765149PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Protocadherin-11 X-linked (PCDH11X), partial CSB-YP765150EQV
CSB-EP765150EQV
CSB-BP765150EQV
CSB-MP765150EQV
CSB-EP765150EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease KN3 CSB-YP765151VDN
CSB-EP765151VDN
CSB-BP765151VDN
CSB-MP765151VDN
CSB-EP765151VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Snake venom serine protease KN6 CSB-YP765152VDN
CSB-EP765152VDN
CSB-BP765152VDN
CSB-MP765152VDN
CSB-EP765152VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viridovipera stejnegeri Stejaggregin-A subunit beta-3 CSB-YP765153VDN
CSB-EP765153VDN
CSB-BP765153VDN
CSB-MP765153VDN
CSB-EP765153VDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Inversin-A (invs-a), partial CSB-YP765154XBE
CSB-EP765154XBE
CSB-BP765154XBE
CSB-MP765154XBE
CSB-EP765154XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Lysosomal-associated transmembrane protein 4B (LAPTM4B), partial CSB-YP765155BO1
CSB-EP765155BO1
CSB-BP765155BO1
CSB-MP765155BO1
CSB-EP765155BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat NEDD8 (Nedd8) CSB-YP765156RA
CSB-EP765156RA
CSB-BP765156RA
CSB-MP765156RA
CSB-EP765156RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sminthopsis hirtipes Sperm protamine P1 (PRM1) CSB-YP765157SHAI
CSB-EP765157SHAI
CSB-BP765157SHAI
CSB-MP765157SHAI
CSB-EP765157SHAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Perca flavescens Calmodulin (calm) CSB-YP765158PFQ
CSB-EP765158PFQ
CSB-BP765158PFQ
CSB-MP765158PFQ
CSB-EP765158PFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antechinus melanurus Sperm protamine P1 (PRM1) CSB-YP765159ABAK
CSB-EP765159ABAK
CSB-BP765159ABAK
CSB-MP765159ABAK
CSB-EP765159ABAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasyuroides byrnei Sperm protamine P1 (PRM1) CSB-YP765160DBAC
CSB-EP765160DBAC
CSB-BP765160DBAC
CSB-MP765160DBAC
CSB-EP765160DBAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L34 (rpmH) CSB-YP765161LAAD
CSB-EP765161LAAD
CSB-BP765161LAAD
CSB-MP765161LAAD
CSB-EP765161LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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