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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L11 (rplK) CSB-YP765222LAAD
CSB-EP765222LAAD
CSB-BP765222LAAD
CSB-MP765222LAAD
CSB-EP765222LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 33 kDa chaperonin (hslO) CSB-YP765223LAAD
CSB-EP765223LAAD
CSB-BP765223LAAD
CSB-MP765223LAAD
CSB-EP765223LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L25 (rplY) CSB-YP765224LAAD
CSB-EP765224LAAD
CSB-BP765224LAAD
CSB-MP765224LAAD
CSB-EP765224LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative septation protein spoVG 2 (spoVG2) CSB-YP765225LAAD
CSB-EP765225LAAD
CSB-BP765225LAAD
CSB-MP765225LAAD
CSB-EP765225LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0213 protein LMOf2365_0181 (LMOf2365_0181) CSB-YP765226LAAD
CSB-EP765226LAAD
CSB-BP765226LAAD
CSB-MP765226LAAD
CSB-EP765226LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative agmatine deiminase 2 (aguA2) CSB-YP765227LAAD
CSB-EP765227LAAD
CSB-BP765227LAAD
CSB-MP765227LAAD
CSB-EP765227LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Adenylosuccinate synthetase (purA) CSB-YP765228DDH
CSB-EP765228DDH
CSB-BP765228DDH
CSB-MP765228DDH
CSB-EP765228DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP765229DDH
CSB-EP765229DDH
CSB-BP765229DDH
CSB-MP765229DDH
CSB-EP765229DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Non-canonical purine NTP pyrophosphatase (DVU_3154) CSB-YP765230DDH
CSB-EP765230DDH
CSB-BP765230DDH
CSB-MP765230DDH
CSB-EP765230DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 3-isopropylmalate dehydrogenase (leuB) CSB-YP765231DDH
CSB-EP765231DDH
CSB-BP765231DDH
CSB-MP765231DDH
CSB-EP765231DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Release factor glutamine methyltransferase (prmC) CSB-YP765232DDH
CSB-EP765232DDH
CSB-BP765232DDH
CSB-MP765232DDH
CSB-EP765232DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Aspartate carbamoyltransferase (pyrB) CSB-YP765233DDH
CSB-EP765233DDH
CSB-BP765233DDH
CSB-MP765233DDH
CSB-EP765233DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 7-cyano-7-deazaguanine synthase (queC) CSB-YP765234DDH
CSB-EP765234DDH
CSB-BP765234DDH
CSB-MP765234DDH
CSB-EP765234DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 50S ribosomal protein L35 (rpmI) CSB-YP765235DDH
CSB-EP765235DDH
CSB-BP765235DDH
CSB-MP765235DDH
CSB-EP765235DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP765236DDH
CSB-EP765236DDH
CSB-BP765236DDH
CSB-MP765236DDH
CSB-EP765236DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris S-adenosylmethionine synthase (metK) CSB-YP765237DDH
CSB-EP765237DDH
CSB-BP765237DDH
CSB-MP765237DDH
CSB-EP765237DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP765238DDH
CSB-EP765238DDH
CSB-BP765238DDH
CSB-MP765238DDH
CSB-EP765238DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP765239DDH
CSB-EP765239DDH
CSB-BP765239DDH
CSB-MP765239DDH
CSB-EP765239DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Tryptophanase (tnaA) CSB-YP765240DDH
CSB-EP765240DDH
CSB-BP765240DDH
CSB-MP765240DDH
CSB-EP765240DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris 60 kDa chaperonin (groL), partial CSB-YP765241DDH
CSB-EP765241DDH
CSB-BP765241DDH
CSB-MP765241DDH
CSB-EP765241DDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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