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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii Ubiquitin-like modifier-activating enzyme ATG7 (ATG7), partial CSB-YP765622DOT
CSB-EP765622DOT
CSB-BP765622DOT
CSB-MP765622DOT
CSB-EP765622DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Adenylyltransferase and sulfurtransferase UBA4 (UBA4) CSB-YP765623DOT
CSB-EP765623DOT
CSB-BP765623DOT
CSB-MP765623DOT
CSB-EP765623DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Succinate dehydrogenase assembly factor 2, mitochondrial (EMI5), partial CSB-YP765624DOT
CSB-EP765624DOT
CSB-BP765624DOT
CSB-MP765624DOT
CSB-EP765624DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Autophagy-related protein 23 (ATG23) CSB-YP765625DOT
CSB-EP765625DOT
CSB-BP765625DOT
CSB-MP765625DOT
CSB-EP765625DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein LOT5 (LOT5) CSB-YP765626DOT
CSB-EP765626DOT
CSB-BP765626DOT
CSB-MP765626DOT
CSB-EP765626DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii SWR1-complex protein 7 (SWC7) CSB-YP765627DOT
CSB-EP765627DOT
CSB-BP765627DOT
CSB-MP765627DOT
CSB-EP765627DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii GPI mannosyltransferase 2 (GPI18), partial CSB-YP765628DOT
CSB-EP765628DOT
CSB-BP765628DOT
CSB-MP765628DOT
CSB-EP765628DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Phosphoglycerate kinase (PGK1) CSB-YP765629DOT
CSB-EP765629DOT
CSB-BP765629DOT
CSB-MP765629DOT
CSB-EP765629DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase DBP10 (DBP10), partial CSB-YP765630DOT
CSB-EP765630DOT
CSB-BP765630DOT
CSB-MP765630DOT
CSB-EP765630DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Autophagy-related protein 27 (ATG27), partial CSB-YP765631DOT
CSB-EP765631DOT
CSB-BP765631DOT
CSB-MP765631DOT
CSB-EP765631DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial import inner membrane translocase subunit TIM54 (TIM54), partial CSB-YP765632DOT1
CSB-EP765632DOT1
CSB-BP765632DOT1
CSB-MP765632DOT1
CSB-EP765632DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Glucose-6-phosphate isomerase (PGI1), partial CSB-YP765633DOT
CSB-EP765633DOT
CSB-BP765633DOT
CSB-MP765633DOT
CSB-EP765633DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Myosin-1 (MYO1), partial CSB-YP765634DOT
CSB-EP765634DOT
CSB-BP765634DOT
CSB-MP765634DOT
CSB-EP765634DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-mRNA-splicing factor SNT309 (SNT309) CSB-YP765635DOT
CSB-EP765635DOT
CSB-BP765635DOT
CSB-MP765635DOT
CSB-EP765635DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Peroxisome assembly protein 22 (PEX22), partial CSB-YP765636DOT1
CSB-EP765636DOT1
CSB-BP765636DOT1
CSB-MP765636DOT1
CSB-EP765636DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pyruvate kinase (PYK1), partial CSB-YP765637DOT
CSB-EP765637DOT
CSB-BP765637DOT
CSB-MP765637DOT
CSB-EP765637DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone chaperone ASF1 (ASF1) CSB-YP765638DOT
CSB-EP765638DOT
CSB-BP765638DOT
CSB-MP765638DOT
CSB-EP765638DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Calpain-like protease palB/RIM13 (RIM13), partial CSB-YP765639DOT
CSB-EP765639DOT
CSB-BP765639DOT
CSB-MP765639DOT
CSB-EP765639DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Cofilin (COF1) CSB-YP765640DOT
CSB-EP765640DOT
CSB-BP765640DOT
CSB-MP765640DOT
CSB-EP765640DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Dynein light chain 1, cytoplasmic (DYN2) CSB-YP765641DOT
CSB-EP765641DOT
CSB-BP765641DOT
CSB-MP765641DOT
CSB-EP765641DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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