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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ashbya gossypii Nuclear distribution protein PAC1 (PAC1) CSB-YP765642DOT
CSB-EP765642DOT
CSB-BP765642DOT
CSB-MP765642DOT
CSB-EP765642DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii pH-response regulator protein palI/RIM9 (RIM9), partial CSB-YP765643DOT1
CSB-EP765643DOT1
CSB-BP765643DOT1
CSB-MP765643DOT1
CSB-EP765643DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii tRNA (guanine (9)-N1)-methyltransferase (TRM10) CSB-YP765644DOT
CSB-EP765644DOT
CSB-BP765644DOT
CSB-MP765644DOT
CSB-EP765644DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Tubulin gamma chain (TUB4) CSB-YP765645DOT
CSB-EP765645DOT
CSB-BP765645DOT
CSB-MP765645DOT
CSB-EP765645DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial inner membrane magnesium transporter LPE10 (LPE10), partial CSB-YP765646DOT1
CSB-EP765646DOT1
CSB-BP765646DOT1
CSB-MP765646DOT1
CSB-EP765646DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ubiquitin-related modifier 1 (URM1) CSB-YP765647DOT
CSB-EP765647DOT
CSB-BP765647DOT
CSB-MP765647DOT
CSB-EP765647DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 60S ribosomal protein L41 (RPL41) CSB-YP765648DOT
CSB-EP765648DOT
CSB-BP765648DOT
CSB-MP765648DOT
CSB-EP765648DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii NADH-cytochrome b5 reductase 1 (CBR1), partial CSB-YP765649DOT
CSB-EP765649DOT
CSB-BP765649DOT
CSB-MP765649DOT
CSB-EP765649DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Maintenance of telomere capping protein 6 (MTC6), partial CSB-YP765650DOT
CSB-EP765650DOT
CSB-BP765650DOT
CSB-MP765650DOT
CSB-EP765650DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii COP9 signalosome complex subunit 10 (RRI2), partial CSB-YP765651DOT
CSB-EP765651DOT
CSB-BP765651DOT
CSB-MP765651DOT
CSB-EP765651DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein FYV10 (FYV10), partial CSB-YP765652DOT
CSB-EP765652DOT
CSB-BP765652DOT
CSB-MP765652DOT
CSB-EP765652DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Putative tyrosine-protein phosphatase OCA1 (OCA1) CSB-YP765653DOT
CSB-EP765653DOT
CSB-BP765653DOT
CSB-MP765653DOT
CSB-EP765653DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Autophagy-related protein 20 (ATG20), partial CSB-YP765654DOT
CSB-EP765654DOT
CSB-BP765654DOT
CSB-MP765654DOT
CSB-EP765654DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Exocyst complex component EXO84 (EXO84), partial CSB-YP765655DOT
CSB-EP765655DOT
CSB-BP765655DOT
CSB-MP765655DOT
CSB-EP765655DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mannose 6-phosphate receptor-like protein 1 (MRL1), partial CSB-YP765656DOT
CSB-EP765656DOT
CSB-BP765656DOT
CSB-MP765656DOT
CSB-EP765656DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein LDB19 (LDB19), partial CSB-YP765657DOT
CSB-EP765657DOT
CSB-BP765657DOT
CSB-MP765657DOT
CSB-EP765657DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase eIF4A (TIF1) CSB-YP765658DOT
CSB-EP765658DOT
CSB-BP765658DOT
CSB-MP765658DOT
CSB-EP765658DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein transport protein SEC13 (SEC13) CSB-YP765659DOT
CSB-EP765659DOT
CSB-BP765659DOT
CSB-MP765659DOT
CSB-EP765659DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Saccharopine dehydrogenase [NAD (+), L-lysine-forming] (LYS1) CSB-YP765660DOT
CSB-EP765660DOT
CSB-BP765660DOT
CSB-MP765660DOT
CSB-EP765660DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Autophagy protein 5 (ATG5) CSB-YP765661DOT
CSB-EP765661DOT
CSB-BP765661DOT
CSB-MP765661DOT
CSB-EP765661DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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