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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii Protein BUR2 (BUR2) CSB-YP765582DOT
CSB-EP765582DOT
CSB-BP765582DOT
CSB-MP765582DOT
CSB-EP765582DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Suppressor of hydroxyurea sensitivity protein 2 (SHU2) CSB-YP765583DOT
CSB-EP765583DOT
CSB-BP765583DOT
CSB-MP765583DOT
CSB-EP765583DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein CSF1 (CSF1), partial CSB-YP765584DOT
CSB-EP765584DOT
CSB-BP765584DOT
CSB-MP765584DOT
CSB-EP765584DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype C Gag polyprotein (gag), partial CSB-YP765585HDAH
CSB-EP765585HDAH
CSB-BP765585HDAH
CSB-MP765585HDAH
CSB-EP765585HDAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype C Protein Nef (nef) CSB-YP765586HDAH
CSB-EP765586HDAH
CSB-BP765586HDAH
CSB-MP765586HDAH
CSB-EP765586HDAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Delta-aminolevulinic acid dehydratase (HEM2) CSB-YP765587DOT
CSB-EP765587DOT
CSB-BP765587DOT
CSB-MP765587DOT
CSB-EP765587DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii UDP-N-acetylglucosamine transferase subunit ALG13 (ALG13) CSB-YP765588DOT
CSB-EP765588DOT
CSB-BP765588DOT
CSB-MP765588DOT
CSB-EP765588DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DASH complex subunit SPC19 (SPC19) CSB-YP765589DOT
CSB-EP765589DOT
CSB-BP765589DOT
CSB-MP765589DOT
CSB-EP765589DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii COP9 signalosome complex subunit 9 (CSN9) CSB-YP765590DOT
CSB-EP765590DOT
CSB-BP765590DOT
CSB-MP765590DOT
CSB-EP765590DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Ribosomal N-lysine methyltransferase 5 (RKM5) CSB-YP765591DOT
CSB-EP765591DOT
CSB-BP765591DOT
CSB-MP765591DOT
CSB-EP765591DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein ATC1/LIC4 (ATC1) CSB-YP765592DOT
CSB-EP765592DOT
CSB-BP765592DOT
CSB-MP765592DOT
CSB-EP765592DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Nascent polypeptide-associated complex subunit beta (EGD1) CSB-YP765593DOT
CSB-EP765593DOT
CSB-BP765593DOT
CSB-MP765593DOT
CSB-EP765593DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 60S ribosomal protein L32 (RPL32) CSB-YP765594DOT
CSB-EP765594DOT
CSB-BP765594DOT
CSB-MP765594DOT
CSB-EP765594DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Putative ATP synthase protein 8-like protein (AGL367W-A), partial CSB-YP765595DOT
CSB-EP765595DOT
CSB-BP765595DOT
CSB-MP765595DOT
CSB-EP765595DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Spindle pole body component KRE28 (KRE28) CSB-YP765596DOT
CSB-EP765596DOT
CSB-BP765596DOT
CSB-MP765596DOT
CSB-EP765596DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Sterol 3-beta-glucosyltransferase (ATG26), partial CSB-YP765597DOT
CSB-EP765597DOT
CSB-BP765597DOT
CSB-MP765597DOT
CSB-EP765597DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mannose-1-phosphate guanyltransferase (MPG1) CSB-YP765598DOT
CSB-EP765598DOT
CSB-BP765598DOT
CSB-MP765598DOT
CSB-EP765598DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Carbamoyl-phosphate synthase arginine-specific small chain (CPA1) CSB-YP765599DOT
CSB-EP765599DOT
CSB-BP765599DOT
CSB-MP765599DOT
CSB-EP765599DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii rRNA-processing protein CGR1 (CGR1) CSB-YP765600DOT
CSB-EP765600DOT
CSB-BP765600DOT
CSB-MP765600DOT
CSB-EP765600DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii D-arabinono-1,4-lactone oxidase (ALO1), partial CSB-YP765601DOT
CSB-EP765601DOT
CSB-BP765601DOT
CSB-MP765601DOT
CSB-EP765601DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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