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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Gloeobacter violaceus tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP766042GCI
CSB-EP766042GCI
CSB-BP766042GCI
CSB-MP766042GCI
CSB-EP766042GCI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum tRNA pseudouridine synthase B (truB) CSB-YP766043CKA
CSB-EP766043CKA
CSB-BP766043CKA
CSB-MP766043CKA
CSB-EP766043CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Probable chemoreceptor glutamine deamidase CheD (cheD) CSB-YP766044VCQ
CSB-EP766044VCQ
CSB-BP766044VCQ
CSB-MP766044VCQ
CSB-EP766044VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0251 protein VVA1436 (VVA1436) CSB-YP766045VCQ
CSB-EP766045VCQ
CSB-BP766045VCQ
CSB-MP766045VCQ
CSB-EP766045VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Autoinducer 2-binding periplasmic protein luxP (luxP) CSB-YP766046VCQ
CSB-EP766046VCQ
CSB-BP766046VCQ
CSB-MP766046VCQ
CSB-EP766046VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Transketolase 2 (tkt2), partial CSB-YP766047VCQ
CSB-EP766047VCQ
CSB-BP766047VCQ
CSB-MP766047VCQ
CSB-EP766047VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Molybdenum import ATP-binding protein ModC (modC) CSB-YP766048VCQ
CSB-EP766048VCQ
CSB-BP766048VCQ
CSB-MP766048VCQ
CSB-EP766048VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Putative phosphotransferase VVA0515 (VVA0515) CSB-YP766049VCQ
CSB-EP766049VCQ
CSB-BP766049VCQ
CSB-MP766049VCQ
CSB-EP766049VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP766050VCQ
CSB-EP766050VCQ
CSB-BP766050VCQ
CSB-MP766050VCQ
CSB-EP766050VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Putative ABC transporter ATP-binding protein VVA0347 (VVA0347), partial CSB-YP766051VCQ
CSB-EP766051VCQ
CSB-BP766051VCQ
CSB-MP766051VCQ
CSB-EP766051VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC (rlmC) CSB-YP766052VCQ
CSB-EP766052VCQ
CSB-BP766052VCQ
CSB-MP766052VCQ
CSB-EP766052VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP766053VCQ
CSB-EP766053VCQ
CSB-BP766053VCQ
CSB-MP766053VCQ
CSB-EP766053VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP766054VCQ
CSB-EP766054VCQ
CSB-BP766054VCQ
CSB-MP766054VCQ
CSB-EP766054VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP synthase epsilon chain (atpC) CSB-YP766055VCQ
CSB-EP766055VCQ
CSB-BP766055VCQ
CSB-MP766055VCQ
CSB-EP766055VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus N5-carboxyaminoimidazole ribonucleotide synthase (purK) CSB-YP766056VCQ
CSB-EP766056VCQ
CSB-BP766056VCQ
CSB-MP766056VCQ
CSB-EP766056VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus UPF0276 protein VV3194 (VV3194) CSB-YP766057VCQ
CSB-EP766057VCQ
CSB-BP766057VCQ
CSB-MP766057VCQ
CSB-EP766057VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP766058VCQ
CSB-EP766058VCQ
CSB-BP766058VCQ
CSB-MP766058VCQ
CSB-EP766058VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 6-phosphofructokinase (pfkA) CSB-YP766059VCQ
CSB-EP766059VCQ
CSB-BP766059VCQ
CSB-MP766059VCQ
CSB-EP766059VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein-export protein SecB (secB) CSB-YP766060VCQ
CSB-EP766060VCQ
CSB-BP766060VCQ
CSB-MP766060VCQ
CSB-EP766060VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Flavohemoprotein (hmp) CSB-YP766061VCQ
CSB-EP766061VCQ
CSB-BP766061VCQ
CSB-MP766061VCQ
CSB-EP766061VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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