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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio Dysbindin-A (dtnbp1a) CSB-YP767244DIL
CSB-EP767244DIL
CSB-BP767244DIL
CSB-MP767244DIL
CSB-EP767244DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio GSK3-beta interaction protein (gskip) CSB-YP767245DIL
CSB-EP767245DIL
CSB-BP767245DIL
CSB-MP767245DIL
CSB-EP767245DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis FERM domain-containing protein 8 (frmd8) CSB-YP767246XBE
CSB-EP767246XBE
CSB-BP767246XBE
CSB-MP767246XBE
CSB-EP767246XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein NDRG1-B (ndrg1-b) CSB-YP767247XBE
CSB-EP767247XBE
CSB-BP767247XBE
CSB-MP767247XBE
CSB-EP767247XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Speckle-type POZ protein A (spop-a) CSB-YP767248XBE
CSB-EP767248XBE
CSB-BP767248XBE
CSB-MP767248XBE
CSB-EP767248XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0452 protein C7orf41 homolog CSB-YP767249XBE
CSB-EP767249XBE
CSB-BP767249XBE
CSB-MP767249XBE
CSB-EP767249XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform (ppp2r2d) CSB-YP767250XBE
CSB-EP767250XBE
CSB-BP767250XBE
CSB-MP767250XBE
CSB-EP767250XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ragulator complex protein LAMTOR2-B (lamtor2-b) CSB-YP767251XBE
CSB-EP767251XBE
CSB-BP767251XBE
CSB-MP767251XBE
CSB-EP767251XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Myotubularin-related protein 4 (mtmr4), partial CSB-YP767252XBE
CSB-EP767252XBE
CSB-BP767252XBE
CSB-MP767252XBE
CSB-EP767252XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Alpha-endosulfine (ensa) CSB-YP767253XBE
CSB-EP767253XBE
CSB-BP767253XBE
CSB-MP767253XBE
CSB-EP767253XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein (herpud2), partial CSB-YP767254XBE1
CSB-EP767254XBE1
CSB-BP767254XBE1
CSB-MP767254XBE1
CSB-EP767254XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Calcitonin gene-related peptide type 1 receptor (calcrl), partial CSB-YP767255XBE1
CSB-EP767255XBE1
CSB-BP767255XBE1
CSB-MP767255XBE1
CSB-EP767255XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ribosome biogenesis protein bop1-B (bop1-b), partial CSB-YP767256XBE
CSB-EP767256XBE
CSB-BP767256XBE
CSB-MP767256XBE
CSB-EP767256XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Eukaryotic translation initiation factor 2A (eif2a), partial CSB-YP767257XBE
CSB-EP767257XBE
CSB-BP767257XBE
CSB-MP767257XBE
CSB-EP767257XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis ATP-dependent RNA helicase DDX42 (ddx42), partial CSB-YP767258XBE
CSB-EP767258XBE
CSB-BP767258XBE
CSB-MP767258XBE
CSB-EP767258XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Apoptosis inhibitor 5-B (api5-b), partial CSB-YP767259XBE
CSB-EP767259XBE
CSB-BP767259XBE
CSB-MP767259XBE
CSB-EP767259XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein CDV3 homolog A (cdv3-a) CSB-YP767260XBE
CSB-EP767260XBE
CSB-BP767260XBE
CSB-MP767260XBE
CSB-EP767260XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Osteoclast-stimulating factor 1 (ostf1) CSB-YP767261XBE
CSB-EP767261XBE
CSB-BP767261XBE
CSB-MP767261XBE
CSB-EP767261XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Kinesin-like protein KIF22-B (kif22-b), partial CSB-YP767262XBE
CSB-EP767262XBE
CSB-BP767262XBE
CSB-MP767262XBE
CSB-EP767262XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 60S ribosomal protein L19 (rpl19) CSB-YP767263XBE
CSB-EP767263XBE
CSB-BP767263XBE
CSB-MP767263XBE
CSB-EP767263XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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