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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Probable inorganic phosphate transporter 1-5 (PHT1-5), partial CSB-YP767103OFG
CSB-EP767103OFG
CSB-BP767103OFG
CSB-MP767103OFG
CSB-EP767103OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Larrea tridentata Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic (RCA1) CSB-YP767104LDAA
CSB-EP767104LDAA
CSB-BP767104LDAA
CSB-MP767104LDAA
CSB-EP767104LDAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Portulaca grandiflora 4,5-DOPA dioxygenase extradiol (DODA) CSB-YP767105PIAD
CSB-BP767105PIAD
CSB-MP767105PIAD
CSB-EP767105PIAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase SINA-like 5 (At1g66660) CSB-YP767106DOA
CSB-EP767106DOA
CSB-BP767106DOA
CSB-MP767106DOA
CSB-EP767106DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Transcription factor LAX PANICLE (LAX) CSB-YP767107OFG
CSB-EP767107OFG
CSB-BP767107OFG
CSB-MP767107OFG
CSB-EP767107OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cyclin-P4-1 (CYCP4-1) CSB-YP767108OFG
CSB-EP767108OFG
CSB-BP767108OFG
CSB-MP767108OFG
CSB-EP767108OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative ripening-related protein 6 (Os10g0489301, Os10g0489300, LOC_Os10g34760) CSB-YP767109OFG
CSB-EP767109OFG
CSB-BP767109OFG
CSB-MP767109OFG
CSB-EP767109OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Soluble starch synthase 2-1, chloroplastic/amyloplastic (SSII-1), partial CSB-YP767110OFG
CSB-EP767110OFG
CSB-BP767110OFG
CSB-MP767110OFG
CSB-EP767110OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative B3 domain-containing protein Os10g0158600 (Os10g0158600, LOC_Os10g07060) CSB-YP767111OFG
CSB-EP767111OFG
CSB-BP767111OFG
CSB-MP767111OFG
CSB-EP767111OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Tyrosine decarboxylase 1 (Os07g0437500, LOC_Os07g25590) CSB-YP767112OFG
CSB-EP767112OFG
CSB-BP767112OFG
CSB-MP767112OFG
CSB-EP767112OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable histone-arginine methyltransferase CARM1 (CARM1), partial CSB-YP767113OFG
CSB-EP767113OFG
CSB-BP767113OFG
CSB-MP767113OFG
CSB-EP767113OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Non-specific lipid-transfer protein 2A (LTP2-A) CSB-YP767114OFG
CSB-EP767114OFG
CSB-BP767114OFG
CSB-MP767114OFG
CSB-EP767114OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Agamous-like MADS-box protein AGL90 (AGL90) CSB-YP767115DOA
CSB-EP767115DOA
CSB-BP767115DOA
CSB-MP767115DOA
CSB-EP767115DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable anion transporter 3, chloroplastic (ANTR3), partial CSB-YP767116DOA
CSB-EP767116DOA
CSB-BP767116DOA
CSB-MP767116DOA
CSB-EP767116DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative B3 domain-containing protein Os04g0676650 (Os04g0676650, Os04g0676500, LOC_Os04g58010) CSB-YP767117OFG
CSB-EP767117OFG
CSB-BP767117OFG
CSB-MP767117OFG
CSB-EP767117OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Auxin response factor 10 (ARF10), partial CSB-YP767118OFG
CSB-EP767118OFG
CSB-BP767118OFG
CSB-MP767118OFG
CSB-EP767118OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc transporter 3 (ZIP3), partial CSB-YP767119OFG
CSB-EP767119OFG
CSB-BP767119OFG
CSB-MP767119OFG
CSB-EP767119OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable RNA-dependent RNA polymerase 2 (RDR2), partial CSB-YP767120OFG
CSB-EP767120OFG
CSB-BP767120OFG
CSB-MP767120OFG
CSB-EP767120OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 37 (Os04g0167900, LOC_Os04g08560) CSB-YP767121OFG
CSB-EP767121OFG
CSB-BP767121OFG
CSB-MP767121OFG
CSB-EP767121OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable potassium transporter 15 (HAK15), partial CSB-YP767122OFG
CSB-EP767122OFG
CSB-BP767122OFG
CSB-MP767122OFG
CSB-EP767122OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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