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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xylella fastidiosa UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP768307XAT
CSB-EP768307XAT
CSB-BP768307XAT
CSB-MP768307XAT
CSB-EP768307XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP768308XAT
CSB-EP768308XAT
CSB-BP768308XAT
CSB-MP768308XAT
CSB-EP768308XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 8-amino-7-oxononanoate synthase (bioF) CSB-YP768309XAT
CSB-EP768309XAT
CSB-BP768309XAT
CSB-MP768309XAT
CSB-EP768309XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP768310XAT
CSB-EP768310XAT
CSB-BP768310XAT
CSB-MP768310XAT
CSB-EP768310XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP768311XAT
CSB-EP768311XAT
CSB-BP768311XAT
CSB-MP768311XAT
CSB-EP768311XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0250 protein PD_0532 (PD_0532) CSB-YP768312XAT
CSB-EP768312XAT
CSB-BP768312XAT
CSB-MP768312XAT
CSB-EP768312XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Malate dehydrogenase (mdh) CSB-YP768313XAT
CSB-EP768313XAT
CSB-BP768313XAT
CSB-MP768313XAT
CSB-EP768313XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S4 (rpsD) CSB-YP768314XAT
CSB-EP768314XAT
CSB-BP768314XAT
CSB-MP768314XAT
CSB-EP768314XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S14 (rpsN) CSB-YP768315XAT
CSB-EP768315XAT
CSB-BP768315XAT
CSB-MP768315XAT
CSB-EP768315XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L16 (rplP) CSB-YP768316XAT
CSB-EP768316XAT
CSB-BP768316XAT
CSB-MP768316XAT
CSB-EP768316XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa ATP synthase subunit delta (atpH) CSB-YP768317XAT
CSB-EP768317XAT
CSB-BP768317XAT
CSB-MP768317XAT
CSB-EP768317XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Transcription elongation factor GreA (greA) CSB-YP768318XAT
CSB-EP768318XAT
CSB-BP768318XAT
CSB-MP768318XAT
CSB-EP768318XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP768319XAT
CSB-EP768319XAT
CSB-BP768319XAT
CSB-MP768319XAT
CSB-EP768319XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP768320XAT
CSB-EP768320XAT
CSB-BP768320XAT
CSB-MP768320XAT
CSB-EP768320XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribonuclease HII (rnhB) CSB-YP768321XAT
CSB-EP768321XAT
CSB-BP768321XAT
CSB-MP768321XAT
CSB-EP768321XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP768322XAT
CSB-EP768322XAT
CSB-BP768322XAT
CSB-MP768322XAT
CSB-EP768322XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP768323XAT
CSB-EP768323XAT
CSB-BP768323XAT
CSB-MP768323XAT
CSB-EP768323XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Adenylate kinase (adk) CSB-YP768324XAT
CSB-EP768324XAT
CSB-BP768324XAT
CSB-MP768324XAT
CSB-EP768324XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosome maturation factor RimP (rimP) CSB-YP768325XAT
CSB-EP768325XAT
CSB-BP768325XAT
CSB-MP768325XAT
CSB-EP768325XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Queuine tRNA-ribosyltransferase (tgt) CSB-YP768326XAT
CSB-EP768326XAT
CSB-BP768326XAT
CSB-MP768326XAT
CSB-EP768326XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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