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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Oryza sativa subsp. japonica Cysteine protease ATG4B (ATG4B) CSB-YP767123OFG
CSB-EP767123OFG
CSB-BP767123OFG
CSB-MP767123OFG
CSB-EP767123OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Serine/threonine-protein kinase SAPK7 (SAPK7) CSB-YP767124OFG
CSB-EP767124OFG
CSB-BP767124OFG
CSB-MP767124OFG
CSB-EP767124OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Methylthioribose kinase 2 (MTK2) CSB-YP767125OFG
CSB-EP767125OFG
CSB-BP767125OFG
CSB-MP767125OFG
CSB-EP767125OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Membrane-anchored ubiquitin-fold protein 4 (MUB4) CSB-YP767126OFG
CSB-EP767126OFG
CSB-BP767126OFG
CSB-MP767126OFG
CSB-EP767126OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative zinc finger CCCH domain-containing protein 29 (Os04g0487500, LOC_Os04g41060) CSB-YP767127OFG
CSB-EP767127OFG
CSB-BP767127OFG
CSB-MP767127OFG
CSB-EP767127OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Thioredoxin-like 3-3 (Os04g0560200, LOC_Os04g47260) CSB-YP767128OFG
CSB-EP767128OFG
CSB-BP767128OFG
CSB-MP767128OFG
CSB-EP767128OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Expansin-B5 (EXPB5) CSB-YP767129OFG
CSB-EP767129OFG
CSB-BP767129OFG
CSB-MP767129OFG
CSB-EP767129OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Copper transporter 6 (COPT6), partial CSB-YP767130OFG1
CSB-EP767130OFG1
CSB-BP767130OFG1
CSB-MP767130OFG1
CSB-EP767130OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica E3 ubiquitin-protein ligase BRE1-like 1 (BRE1A), partial CSB-YP767131OFG
CSB-EP767131OFG
CSB-BP767131OFG
CSB-MP767131OFG
CSB-EP767131OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable aquaporin PIP2-3 (PIP2-3), partial CSB-YP767132OFG1
CSB-EP767132OFG1
CSB-BP767132OFG1
CSB-MP767132OFG1
CSB-EP767132OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Homeobox-leucine zipper protein HOX22 (HOX22) CSB-YP767133OFG
CSB-EP767133OFG
CSB-BP767133OFG
CSB-MP767133OFG
CSB-EP767133OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Formin-like protein 2 (FH2), partial CSB-YP767134OFG
CSB-EP767134OFG
CSB-BP767134OFG
CSB-MP767134OFG
CSB-EP767134OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein phosphatase 2C 39 (Os04g0403701, LOC_Os04g33080), partial CSB-YP767135OFG
CSB-EP767135OFG
CSB-BP767135OFG
CSB-MP767135OFG
CSB-EP767135OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Griffithsia japonica Photosystem II 12 kDa extrinsic protein, chloroplastic (psbU) CSB-YP767136GCAL
CSB-EP767136GCAL
CSB-BP767136GCAL
CSB-MP767136GCAL
CSB-EP767136GCAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemisia spiciformis Farnesyl diphosphate synthase 1 (FDS-1) CSB-YP767137AGAL
CSB-EP767137AGAL
CSB-BP767137AGAL
CSB-MP767137AGAL
CSB-EP767137AGAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative germin-like protein 3-2 (Os03g0651800, LOC_Os03g44880) CSB-YP767138OFG
CSB-EP767138OFG
CSB-BP767138OFG
CSB-MP767138OFG
CSB-EP767138OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Allene oxide synthase 1, chloroplastic (CYP74A1) CSB-YP767139OFG
CSB-EP767139OFG
CSB-BP767139OFG
CSB-MP767139OFG
CSB-EP767139OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Two-component response regulator EHD1 (EHD1) CSB-YP767140OFG
CSB-EP767140OFG
CSB-BP767140OFG
CSB-MP767140OFG
CSB-EP767140OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica DNA (cytosine-5)-methyltransferase 1A (MET1A), partial CSB-YP767141OFG
CSB-EP767141OFG
CSB-BP767141OFG
CSB-MP767141OFG
CSB-EP767141OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g57430, chloroplastic (PCMP-H81), partial CSB-YP767142DOA
CSB-EP767142DOA
CSB-BP767142DOA
CSB-MP767142DOA
CSB-EP767142DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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