Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant NAD-dependent protein deacetylase (cobB) CSB-YP767504BQE
CSB-EP767504BQE
CSB-BP767504BQE
CSB-MP767504BQE
CSB-EP767504BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type II pantothenate kinase (coaW) CSB-YP767505BQE
CSB-EP767505BQE
CSB-BP767505BQE
CSB-MP767505BQE
CSB-EP767505BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP767506BQE
CSB-EP767506BQE
CSB-BP767506BQE
CSB-MP767506BQE
CSB-EP767506BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-alanine--D-alanine ligase (ddl) CSB-YP767507BQE
CSB-EP767507BQE
CSB-BP767507BQE
CSB-MP767507BQE
CSB-EP767507BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stage II sporulation protein SA (spoIISA), partial CSB-YP767508BQE1
CSB-EP767508BQE1
CSB-BP767508BQE1
CSB-MP767508BQE1
CSB-EP767508BQE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sec-independent protein translocase protein TatA (tatA), partial CSB-YP767509BQE
CSB-EP767509BQE
CSB-BP767509BQE
CSB-MP767509BQE
CSB-EP767509BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP767510BQE
CSB-EP767510BQE
CSB-BP767510BQE
CSB-MP767510BQE
CSB-EP767510BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant FMN-dependent NADH-azoreductase 2 (azoR2) CSB-YP767511BQE
CSB-EP767511BQE
CSB-BP767511BQE
CSB-MP767511BQE
CSB-EP767511BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fumarate hydratase class II (fumC) CSB-YP767512BQE
CSB-EP767512BQE
CSB-BP767512BQE
CSB-MP767512BQE
CSB-EP767512BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate 1-decarboxylase (panD) CSB-YP767513BQE
CSB-EP767513BQE
CSB-BP767513BQE
CSB-MP767513BQE
CSB-EP767513BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate reductase (dapB) CSB-YP767514BQE
CSB-EP767514BQE
CSB-BP767514BQE
CSB-MP767514BQE
CSB-EP767514BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP767515BQE
CSB-EP767515BQE
CSB-BP767515BQE
CSB-MP767515BQE
CSB-EP767515BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adapter protein mecA 2 (mecA2) CSB-YP767516BQE
CSB-EP767516BQE
CSB-BP767516BQE
CSB-MP767516BQE
CSB-EP767516BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP phosphoribosyltransferase (hisG) CSB-YP767517BQE
CSB-EP767517BQE
CSB-BP767517BQE
CSB-MP767517BQE
CSB-EP767517BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase 2 (uppP2), partial CSB-YP767518BQE
CSB-EP767518BQE
CSB-BP767518BQE
CSB-MP767518BQE
CSB-EP767518BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP767519BQE
CSB-EP767519BQE
CSB-BP767519BQE
CSB-MP767519BQE
CSB-EP767519BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-oxoglutarate dehydrogenase E1 component (odhA), partial CSB-YP767520BQE
CSB-EP767520BQE
CSB-BP767520BQE
CSB-MP767520BQE
CSB-EP767520BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] (prpE) CSB-YP767521BQE
CSB-EP767521BQE
CSB-BP767521BQE
CSB-MP767521BQE
CSB-EP767521BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein spx 1 (spxA1) CSB-YP767522BQE
CSB-EP767522BQE
CSB-BP767522BQE
CSB-MP767522BQE
CSB-EP767522BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP767523BQE
CSB-EP767523BQE
CSB-BP767523BQE
CSB-MP767523BQE
CSB-EP767523BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>