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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactobacillus plantarum 3-dehydroquinate synthase (aroB) CSB-YP768647LMS
CSB-EP768647LMS
CSB-BP768647LMS
CSB-MP768647LMS
CSB-EP768647LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP768661BTY
CSB-EP768661BTY
CSB-BP768661BTY
CSB-MP768661BTY
CSB-EP768661BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella bronchiseptica tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP768662BTY
CSB-EP768662BTY
CSB-BP768662BTY
CSB-MP768662BTY
CSB-EP768662BTY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus helveticus Beta-galactosidase small subunit (lacM) CSB-YP768663LMP
CSB-EP768663LMP
CSB-BP768663LMP
CSB-MP768663LMP
CSB-EP768663LMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Flavohemoprotein (hmp) CSB-YP768664RKU
CSB-EP768664RKU
CSB-BP768664RKU
CSB-MP768664RKU
CSB-EP768664RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Small, acid-soluble spore protein J (sspJ) CSB-YP768665BRJ
CSB-EP768665BRJ
CSB-BP768665BRJ
CSB-MP768665BRJ
CSB-EP768665BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Small, acid-soluble spore protein L (sspL) CSB-YP768666BRJ
CSB-EP768666BRJ
CSB-BP768666BRJ
CSB-MP768666BRJ
CSB-EP768666BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0296 protein ylzA (ylzA) CSB-YP768667BRJ
CSB-EP768667BRJ
CSB-BP768667BRJ
CSB-MP768667BRJ
CSB-EP768667BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP768668AIY
CSB-EP768668AIY
CSB-BP768668AIY
CSB-MP768668AIY
CSB-EP768668AIY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Oxygen sensor histidine kinase nreB (nreB) CSB-YP768669FLK
CSB-EP768669FLK
CSB-BP768669FLK
CSB-MP768669FLK
CSB-EP768669FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Moritella sp. Malate dehydrogenase (mdh) CSB-YP768670MGAT
CSB-EP768670MGAT
CSB-BP768670MGAT
CSB-MP768670MGAT
CSB-EP768670MGAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L34 (rpmH) CSB-YP768671TNP
CSB-EP768671TNP
CSB-BP768671TNP
CSB-MP768671TNP
CSB-EP768671TNP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Defensin-like protein 99 (At4g22214) CSB-YP768672DOA
CSB-EP768672DOA
CSB-BP768672DOA
CSB-MP768672DOA
CSB-EP768672DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Glutamyl-tRNA (Gln) amidotransferase subunit A, chloroplastic/mitochondrial (GATA), partial CSB-YP768673OFG
CSB-EP768673OFG
CSB-BP768673OFG
CSB-MP768673OFG
CSB-EP768673OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Cytoplasmic dynein 2 light intermediate chain 1 (D1BLIC) CSB-YP768674DSJ
CSB-EP768674DSJ
CSB-BP768674DSJ
CSB-MP768674DSJ
CSB-EP768674DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 2 (MTN2) CSB-YP768675DOA
CSB-EP768675DOA
CSB-BP768675DOA
CSB-MP768675DOA
CSB-EP768675DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Populus tremuloides Isoprene synthase, chloroplastic (ISPS), partial CSB-YP768676EXR
CSB-EP768676EXR
CSB-BP768676EXR
CSB-MP768676EXR
CSB-EP768676EXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Homeobox-leucine zipper protein HOX1 (HOX1) CSB-YP768677OFG
CSB-EP768677OFG
CSB-BP768677OFG
CSB-MP768677OFG
CSB-EP768677OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Malate dehydrogenase, cytoplasmic (Os10g0478200, LOC_Os10g33800) CSB-YP768678OFG
CSB-EP768678OFG
CSB-BP768678OFG
CSB-MP768678OFG
CSB-EP768678OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 4 (ARD4) CSB-YP768679OFG
CSB-EP768679OFG
CSB-BP768679OFG
CSB-MP768679OFG
CSB-EP768679OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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