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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas syringae pv. tomato Dihydrodipicolinate reductase (dapB) CSB-YP768447FGP
CSB-EP768447FGP
CSB-BP768447FGP
CSB-MP768447FGP
CSB-EP768447FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphoglucosamine mutase (glmM) CSB-YP768448FGP
CSB-EP768448FGP
CSB-BP768448FGP
CSB-MP768448FGP
CSB-EP768448FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP768449FGP
CSB-EP768449FGP
CSB-BP768449FGP
CSB-MP768449FGP
CSB-EP768449FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Histidinol-phosphate aminotransferase (hisC) CSB-YP768450FGP
CSB-EP768450FGP
CSB-BP768450FGP
CSB-MP768450FGP
CSB-EP768450FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Protein MraZ (mraZ) CSB-YP768451FGP
CSB-EP768451FGP
CSB-BP768451FGP
CSB-MP768451FGP
CSB-EP768451FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP768452FGP
CSB-EP768452FGP
CSB-BP768452FGP
CSB-MP768452FGP
CSB-EP768452FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Glutamate N-acetyltransferase (argJ) CSB-YP768453FGP
CSB-EP768453FGP
CSB-BP768453FGP
CSB-MP768453FGP
CSB-EP768453FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato DNA repair protein recO (recO) CSB-YP768454FGP
CSB-EP768454FGP
CSB-BP768454FGP
CSB-MP768454FGP
CSB-EP768454FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Acyl-homoserine lactone acylase quiP (quiP), partial CSB-YP768455FGP
CSB-EP768455FGP
CSB-BP768455FGP
CSB-MP768455FGP
CSB-EP768455FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Probable potassium transport system protein kup (kup), partial CSB-YP768456FGP
CSB-EP768456FGP
CSB-BP768456FGP
CSB-MP768456FGP
CSB-EP768456FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP768457FGP
CSB-EP768457FGP
CSB-BP768457FGP
CSB-MP768457FGP
CSB-EP768457FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0745 protein PSPTO_3921 (PSPTO_3921) CSB-YP768458FGP
CSB-EP768458FGP
CSB-BP768458FGP
CSB-MP768458FGP
CSB-EP768458FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Probable septum site-determining protein MinC (minC) CSB-YP768459FGP
CSB-EP768459FGP
CSB-BP768459FGP
CSB-MP768459FGP
CSB-EP768459FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP768460FGP
CSB-EP768460FGP
CSB-BP768460FGP
CSB-MP768460FGP
CSB-EP768460FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Bifunctional protein FolD 1 (folD1) CSB-YP768461FGP
CSB-EP768461FGP
CSB-BP768461FGP
CSB-MP768461FGP
CSB-EP768461FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 1-aminocyclopropane-1-carboxylate deaminase (acdS) CSB-YP768462FGP
CSB-EP768462FGP
CSB-BP768462FGP
CSB-MP768462FGP
CSB-EP768462FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP768463FGP
CSB-EP768463FGP
CSB-BP768463FGP
CSB-MP768463FGP
CSB-EP768463FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato LexA repressor 1 (lexA1) CSB-YP768464FGP
CSB-EP768464FGP
CSB-BP768464FGP
CSB-MP768464FGP
CSB-EP768464FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP768465FGP
CSB-EP768465FGP
CSB-BP768465FGP
CSB-MP768465FGP
CSB-EP768465FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Translation initiation factor IF-1 (infA) CSB-YP768466FGP
CSB-EP768466FGP
CSB-BP768466FGP
CSB-MP768466FGP
CSB-EP768466FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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