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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant UvrABC system protein B (uvrB), partial CSB-YP767965SZB
CSB-EP767965SZB
CSB-BP767965SZB
CSB-MP767965SZB
CSB-EP767965SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0271 protein YbgL (ybgL) CSB-YP767966SZB
CSB-EP767966SZB
CSB-BP767966SZB
CSB-MP767966SZB
CSB-EP767966SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferrochelatase (hemH) CSB-YP767967SZB
CSB-EP767967SZB
CSB-BP767967SZB
CSB-MP767967SZB
CSB-EP767967SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipid-A-disaccharide synthase (lpxB) CSB-YP767968SZB
CSB-EP767968SZB
CSB-BP767968SZB
CSB-MP767968SZB
CSB-EP767968SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator sgrR (sgrR), partial CSB-YP767969SZB
CSB-EP767969SZB
CSB-BP767969SZB
CSB-MP767969SZB
CSB-EP767969SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carnitine operon protein CaiE (caiE) CSB-YP767970SZB
CSB-EP767970SZB
CSB-BP767970SZB
CSB-MP767970SZB
CSB-EP767970SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP767971LGX
CSB-EP767971LGX
CSB-BP767971LGX
CSB-MP767971LGX
CSB-EP767971LGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sarcosine/dimethylglycine N-methyltransferase CSB-YP767972DNJ
CSB-EP767972DNJ
CSB-BP767972DNJ
CSB-MP767972DNJ
CSB-EP767972DNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio harveyi 60 kDa chaperonin 1 (groL1), partial CSB-YP767973VFD
CSB-EP767973VFD
CSB-BP767973VFD
CSB-MP767973VFD
CSB-EP767973VFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oenococcus oeni ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP767974OAO
CSB-EP767974OAO
CSB-BP767974OAO
CSB-MP767974OAO
CSB-EP767974OAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Odontoglossum ringspot virus Replicase large subunit, partial CSB-YP767975OBAL
CSB-EP767975OBAL
CSB-BP767975OBAL
CSB-MP767975OBAL
CSB-EP767975OBAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Orf virus DNA polymerase (POL), partial CSB-YP767976OCE
CSB-EP767976OCE
CSB-BP767976OCE
CSB-MP767976OCE
CSB-EP767976OCE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulatory protein E2 (E2) CSB-YP767977HOM
CSB-EP767977HOM
CSB-BP767977HOM
CSB-MP767977HOM
CSB-EP767977HOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP767978BPS
CSB-EP767978BPS
CSB-BP767978BPS
CSB-MP767978BPS
CSB-EP767978BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Histidinol dehydrogenase (hisD) CSB-YP767979BPS
CSB-EP767979BPS
CSB-BP767979BPS
CSB-MP767979BPS
CSB-EP767979BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pea enation mosaic virus-1 Suppressor of silencing P0 (ORF0) CSB-YP767980PDX
CSB-EP767980PDX
CSB-BP767980PDX
CSB-MP767980PDX
CSB-EP767980PDX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paprika mild mottle virus Capsid protein (CP) CSB-YP767981PIAE
CSB-EP767981PIAE
CSB-BP767981PIAE
CSB-MP767981PIAE
CSB-EP767981PIAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-lactamase SHV-46 (bla) CSB-YP767982KBE
CSB-EP767982KBE
CSB-BP767982KBE
CSB-MP767982KBE
CSB-EP767982KBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable efflux pump membrane transporter SepB (sepB), partial CSB-YP767983PZN
CSB-EP767983PZN
CSB-BP767983PZN
CSB-MP767983PZN
CSB-EP767983PZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus acidophilus Protein grpE (grpE) CSB-YP767984LME
CSB-EP767984LME
CSB-BP767984LME
CSB-MP767984LME
CSB-EP767984LME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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