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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Beta-glucanase (licB) CSB-YP767985CMO
CSB-EP767985CMO
CSB-BP767985CMO
CSB-MP767985CMO
CSB-EP767985CMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemin import ATP-binding protein HmuV (hmuV) CSB-YP767986EKS
CSB-EP767986EKS
CSB-BP767986EKS
CSB-MP767986EKS
CSB-EP767986EKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Phosphoenolpyruvate-protein phosphotransferase (ptsI), partial CSB-YP767987LRU
CSB-EP767987LRU
CSB-BP767987LRU
CSB-MP767987LRU
CSB-EP767987LRU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium extorquens Malate dehydrogenase (mdh) CSB-YP767988MSZ
CSB-EP767988MSZ
CSB-BP767988MSZ
CSB-MP767988MSZ
CSB-EP767988MSZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis Amylosucrase (ams), partial CSB-YP767989NBJ
CSB-EP767989NBJ
CSB-BP767989NBJ
CSB-MP767989NBJ
CSB-EP767989NBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histidinol-phosphate aminotransferase (hisC) CSB-YP767990BXC
CSB-EP767990BXC
CSB-BP767990BXC
CSB-MP767990BXC
CSB-EP767990BXC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP767991BXB
CSB-EP767991BXB
CSB-BP767991BXB
CSB-MP767991BXB
CSB-EP767991BXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 14-3-3-like protein GF14-A (GF14A) CSB-YP767992OFG
CSB-EP767992OFG
CSB-BP767992OFG
CSB-MP767992OFG
CSB-EP767992OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Gibberellin-regulated protein 5 (GASA5) CSB-YP767993DOA
CSB-EP767993DOA
CSB-BP767993DOA
CSB-MP767993DOA
CSB-EP767993DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase SINAT3 (SINAT3) CSB-YP767994DOA
CSB-EP767994DOA
CSB-BP767994DOA
CSB-MP767994DOA
CSB-EP767994DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Homeobox protein knotted-1-like 6 (KNAT6) CSB-YP767995DOA
CSB-EP767995DOA
CSB-BP767995DOA
CSB-MP767995DOA
CSB-EP767995DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase/pectinesterase inhibitor 19 (PME19), partial CSB-YP767996DOA
CSB-EP767996DOA
CSB-BP767996DOA
CSB-MP767996DOA
CSB-EP767996DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana MATE efflux family protein 2, chloroplastic (DTX44), partial CSB-YP767997DOA
CSB-EP767997DOA
CSB-BP767997DOA
CSB-MP767997DOA
CSB-EP767997DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDP-mannose transporter GONST4 (GONST4), partial CSB-YP767998DOA
CSB-EP767998DOA
CSB-BP767998DOA
CSB-MP767998DOA
CSB-EP767998DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Fructan 1-exohydrolase w2 (1-FEHw2), partial CSB-YP767999TQN
CSB-EP767999TQN
CSB-BP767999TQN
CSB-MP767999TQN
CSB-EP767999TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana attenuata Probable 5-epi-aristolochene synthase 4, partial CSB-YP768000NBAZ
CSB-EP768000NBAZ
CSB-BP768000NBAZ
CSB-MP768000NBAZ
CSB-EP768000NBAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Mechanosensitive ion channel protein 9 (MSL9), partial CSB-YP768002DOA1
CSB-EP768002DOA1
CSB-BP768002DOA1
CSB-MP768002DOA1
CSB-EP768002DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG8 (LOG8) CSB-YP768003DOA
CSB-EP768003DOA
CSB-BP768003DOA
CSB-MP768003DOA
CSB-EP768003DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Heat stress transcription factor A-2a (HSFA2A) CSB-YP768004OFG
CSB-EP768004OFG
CSB-BP768004OFG
CSB-MP768004OFG
CSB-EP768004OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays CRS2-associated factor 2, chloroplastic (CAF2), partial CSB-YP768005ZAX
CSB-EP768005ZAX
CSB-BP768005ZAX
CSB-MP768005ZAX
CSB-EP768005ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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