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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Outer membrane protein U (ompU) CSB-YP768367VFE
CSB-EP768367VFE
CSB-BP768367VFE
CSB-MP768367VFE
CSB-EP768367VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP768368VFE
CSB-EP768368VFE
CSB-BP768368VFE
CSB-MP768368VFE
CSB-EP768368VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galactokinase (galK) CSB-YP768369VFE
CSB-EP768369VFE
CSB-BP768369VFE
CSB-MP768369VFE
CSB-EP768369VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Na (+)-translocating NADH-quinone reductase subunit D (nqrD), partial CSB-YP768370VFE1
CSB-EP768370VFE1
CSB-BP768370VFE1
CSB-MP768370VFE1
CSB-EP768370VFE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant [Protein-PII] uridylyltransferase (glnD), partial CSB-YP768371VFE
CSB-EP768371VFE
CSB-BP768371VFE
CSB-MP768371VFE
CSB-EP768371VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP768372VFE
CSB-EP768372VFE
CSB-BP768372VFE
CSB-MP768372VFE
CSB-EP768372VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP768373VFE
CSB-EP768373VFE
CSB-BP768373VFE
CSB-MP768373VFE
CSB-EP768373VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-ketoacyl-CoA thiolase (fadI) CSB-YP768374VFE
CSB-EP768374VFE
CSB-BP768374VFE
CSB-MP768374VFE
CSB-EP768374VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A (truA) CSB-YP768375VFE
CSB-EP768375VFE
CSB-BP768375VFE
CSB-MP768375VFE
CSB-EP768375VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0597 protein VP2173 (VP2173) CSB-YP768376VFE
CSB-EP768376VFE
CSB-BP768376VFE
CSB-MP768376VFE
CSB-EP768376VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Electron transport complex protein RnfD (rnfD), partial CSB-YP768377VFE
CSB-EP768377VFE
CSB-BP768377VFE
CSB-MP768377VFE
CSB-EP768377VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP768378VFE
CSB-EP768378VFE
CSB-BP768378VFE
CSB-MP768378VFE
CSB-EP768378VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Na (+)/H (+) antiporter nhaB (nhaB), partial CSB-YP768379VFE
CSB-EP768379VFE
CSB-BP768379VFE
CSB-MP768379VFE
CSB-EP768379VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate kinase (tmk) CSB-YP768380VFE
CSB-EP768380VFE
CSB-BP768380VFE
CSB-MP768380VFE
CSB-EP768380VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl-homoserine-lactone synthase opaM (opaM) CSB-YP768381VFE
CSB-EP768381VFE
CSB-BP768381VFE
CSB-MP768381VFE
CSB-EP768381VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c-552 (nrfA) CSB-YP768382VFE
CSB-EP768382VFE
CSB-BP768382VFE
CSB-MP768382VFE
CSB-EP768382VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diaminobutyrate--2-oxoglutarate transaminase (ectB) CSB-YP768383VFE
CSB-EP768383VFE
CSB-BP768383VFE
CSB-MP768383VFE
CSB-EP768383VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein ProQ homolog (proQ) CSB-YP768384VFE
CSB-EP768384VFE
CSB-BP768384VFE
CSB-MP768384VFE
CSB-EP768384VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein VP1481 (VP1481) CSB-YP768385VFE
CSB-EP768385VFE
CSB-BP768385VFE
CSB-MP768385VFE
CSB-EP768385VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitamin B12 import ATP-binding protein BtuD (btuD) CSB-YP768386VFE
CSB-EP768386VFE
CSB-BP768386VFE
CSB-MP768386VFE
CSB-EP768386VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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