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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Probable histone-arginine methyltransferase 1.3 (PRMT13), partial CSB-YP769581DOA
CSB-EP769581DOA
CSB-BP769581DOA
CSB-MP769581DOA
CSB-EP769581DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable peptide/nitrate transporter At2g02020 (At2g02020), partial CSB-YP769582DOA
CSB-EP769582DOA
CSB-BP769582DOA
CSB-MP769582DOA
CSB-EP769582DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AP-2 complex subunit sigma (AP17) CSB-YP769583DOA
CSB-EP769583DOA
CSB-BP769583DOA
CSB-MP769583DOA
CSB-EP769583DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CASP-like protein At2g36330 (At2g36330), partial CSB-YP769584DOA1
CSB-EP769584DOA1
CSB-BP769584DOA1
CSB-MP769584DOA1
CSB-EP769584DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Auxin response factor 17 (ARF17), partial CSB-YP769585DOA
CSB-EP769585DOA
CSB-BP769585DOA
CSB-MP769585DOA
CSB-EP769585DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH1 (ASHH1) CSB-YP769586DOA
CSB-EP769586DOA
CSB-BP769586DOA
CSB-MP769586DOA
CSB-EP769586DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dynamin-related protein 1B (DRP1B), partial CSB-YP769587DOA
CSB-EP769587DOA
CSB-BP769587DOA
CSB-MP769587DOA
CSB-EP769587DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Potassium transporter 26 (HAK26), partial CSB-YP769588OFG
CSB-EP769588OFG
CSB-BP769588OFG
CSB-MP769588OFG
CSB-EP769588OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable cellulose synthase A catalytic subunit 8 [UDP-forming] (CESA8), partial CSB-YP769589OFG
CSB-EP769589OFG
CSB-BP769589OFG
CSB-MP769589OFG
CSB-EP769589OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 3 CSB-YP769590RGX
CSB-EP769590RGX
CSB-BP769590RGX
CSB-MP769590RGX
CSB-EP769590RGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Two pore potassium channel a (TPKA), partial CSB-YP769591OFG1
CSB-EP769591OFG1
CSB-BP769591OFG1
CSB-MP769591OFG1
CSB-EP769591OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Ammonium transporter 3 member 2 (AMT3-2), partial CSB-YP769592OFG
CSB-EP769592OFG
CSB-BP769592OFG
CSB-MP769592OFG
CSB-EP769592OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Derlin-2 (DER2), partial CSB-YP769593OFG1
CSB-EP769593OFG1
CSB-BP769593OFG1
CSB-MP769593OFG1
CSB-EP769593OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Piry virus Glycoprotein G (G), partial CSB-YP769594PKU1
CSB-EP769594PKU1
CSB-BP769594PKU1
CSB-MP769594PKU1
CSB-EP769594PKU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Expansin-A7 (EXPA7) CSB-YP769595OFG
CSB-EP769595OFG
CSB-BP769595OFG
CSB-MP769595OFG
CSB-EP769595OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable aldehyde oxidase 2 (Os03g0790900, LOC_Os03g57690), partial CSB-YP769596OFG
CSB-EP769596OFG
CSB-BP769596OFG
CSB-MP769596OFG
CSB-EP769596OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice stripe virus RNA-dependent RNA polymerase (pc1), partial CSB-YP769597REQ
CSB-EP769597REQ
CSB-BP769597REQ
CSB-MP769597REQ
CSB-EP769597REQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice dwarf virus Movement protein, partial CSB-YP769598RCAO
CSB-EP769598RCAO
CSB-BP769598RCAO
CSB-MP769598RCAO
CSB-EP769598RCAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice dwarf virus Protein P7, partial CSB-YP769599REN
CSB-EP769599REN
CSB-BP769599REN
CSB-MP769599REN
CSB-EP769599REN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse mammary tumor virus Envelope glycoprotein gp70 (env), partial CSB-YP769600MIU
CSB-EP769600MIU
CSB-BP769600MIU
CSB-MP769600MIU
CSB-EP769600MIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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