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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida Co-chaperone protein hscB homolog (hscB) CSB-YP768587FGB
CSB-EP768587FGB
CSB-BP768587FGB
CSB-MP768587FGB
CSB-EP768587FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Protoheme IX farnesyltransferase 2 (cyoE2), partial CSB-YP768588FGB
CSB-EP768588FGB
CSB-BP768588FGB
CSB-MP768588FGB
CSB-EP768588FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Uracil phosphoribosyltransferase (upp) CSB-YP768589FGB
CSB-EP768589FGB
CSB-BP768589FGB
CSB-MP768589FGB
CSB-EP768589FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L25 (rplY) CSB-YP768590FGB
CSB-EP768590FGB
CSB-BP768590FGB
CSB-MP768590FGB
CSB-EP768590FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Serine hydroxymethyltransferase 2 (glyA2) CSB-YP768591FGB
CSB-EP768591FGB
CSB-BP768591FGB
CSB-MP768591FGB
CSB-EP768591FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Isoleucine--tRNA ligase (ileS), partial CSB-YP768592FGB
CSB-EP768592FGB
CSB-BP768592FGB
CSB-MP768592FGB
CSB-EP768592FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP768593FGB
CSB-EP768593FGB
CSB-BP768593FGB
CSB-MP768593FGB
CSB-EP768593FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida L-seryl-tRNA (Sec) selenium transferase (selA) CSB-YP768594FGB
CSB-EP768594FGB
CSB-BP768594FGB
CSB-MP768594FGB
CSB-EP768594FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L15 (rplO) CSB-YP768595FGB
CSB-EP768595FGB
CSB-BP768595FGB
CSB-MP768595FGB
CSB-EP768595FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 30S ribosomal protein S14 (rpsN) CSB-YP768596FGB
CSB-EP768596FGB
CSB-BP768596FGB
CSB-MP768596FGB
CSB-EP768596FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L16 (rplP) CSB-YP768597FGB
CSB-EP768597FGB
CSB-BP768597FGB
CSB-MP768597FGB
CSB-EP768597FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L3 (rplC) CSB-YP768598FGB
CSB-EP768598FGB
CSB-BP768598FGB
CSB-MP768598FGB
CSB-EP768598FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP768599FGB
CSB-EP768599FGB
CSB-BP768599FGB
CSB-MP768599FGB
CSB-EP768599FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Type III pantothenate kinase (coaX) CSB-YP768600FGB
CSB-EP768600FGB
CSB-BP768600FGB
CSB-MP768600FGB
CSB-EP768600FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Indole-3-glycerol phosphate synthase (trpC) CSB-YP768601FGB
CSB-EP768601FGB
CSB-BP768601FGB
CSB-MP768601FGB
CSB-EP768601FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Thiosulfate sulfurtransferase glpE (glpE) CSB-YP768602FGB
CSB-EP768602FGB
CSB-BP768602FGB
CSB-MP768602FGB
CSB-EP768602FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Coenzyme PQQ synthesis protein B (pqqB) CSB-YP768603FGB
CSB-EP768603FGB
CSB-BP768603FGB
CSB-MP768603FGB
CSB-EP768603FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Biotin synthase (bioB) CSB-YP768604FGB
CSB-EP768604FGB
CSB-BP768604FGB
CSB-MP768604FGB
CSB-EP768604FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable Fe (2+)-trafficking protein (PP_0285) CSB-YP768605FGB
CSB-EP768605FGB
CSB-BP768605FGB
CSB-MP768605FGB
CSB-EP768605FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Aliphatic sulfonates import ATP-binding protein SsuB (ssuB) CSB-YP768606FGB
CSB-EP768606FGB
CSB-BP768606FGB
CSB-MP768606FGB
CSB-EP768606FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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