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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas syringae pv. tomato Methylthioribulose-1-phosphate dehydratase (mtnB) CSB-YP770061FGP
CSB-EP770061FGP
CSB-BP770061FGP
CSB-MP770061FGP
CSB-EP770061FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Flagellar L-ring protein (flgH) CSB-YP770062FGP
CSB-EP770062FGP
CSB-BP770062FGP
CSB-MP770062FGP
CSB-EP770062FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Acetyl-coenzyme A synthetase (acsA), partial CSB-YP770063FGP
CSB-EP770063FGP
CSB-BP770063FGP
CSB-MP770063FGP
CSB-EP770063FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0176 protein PSPTO_1734 (PSPTO_1734) CSB-YP770064FGP
CSB-EP770064FGP
CSB-BP770064FGP
CSB-MP770064FGP
CSB-EP770064FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Protein sprT (sprT) CSB-YP770065FGP
CSB-EP770065FGP
CSB-BP770065FGP
CSB-MP770065FGP
CSB-EP770065FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Peptide deformylase 2 (def2) CSB-YP770066FGP
CSB-EP770066FGP
CSB-BP770066FGP
CSB-MP770066FGP
CSB-EP770066FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP770067FGP
CSB-EP770067FGP
CSB-BP770067FGP
CSB-MP770067FGP
CSB-EP770067FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato tRNA (Ile)-lysidine synthase (tilS) CSB-YP770068FGP
CSB-EP770068FGP
CSB-BP770068FGP
CSB-MP770068FGP
CSB-EP770068FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP770069FGP
CSB-EP770069FGP
CSB-BP770069FGP
CSB-MP770069FGP
CSB-EP770069FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 30S ribosomal protein S2 (rpsB) CSB-YP770070FGP
CSB-EP770070FGP
CSB-BP770070FGP
CSB-MP770070FGP
CSB-EP770070FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Adenylate kinase (adk) CSB-YP770071FGP
CSB-EP770071FGP
CSB-BP770071FGP
CSB-MP770071FGP
CSB-EP770071FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP770072FGP
CSB-EP770072FGP
CSB-BP770072FGP
CSB-MP770072FGP
CSB-EP770072FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Histidine--tRNA ligase (hisS) CSB-YP770073FGP
CSB-EP770073FGP
CSB-BP770073FGP
CSB-MP770073FGP
CSB-EP770073FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Queuine tRNA-ribosyltransferase (tgt) CSB-YP770074FGP
CSB-EP770074FGP
CSB-BP770074FGP
CSB-MP770074FGP
CSB-EP770074FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Protoheme IX farnesyltransferase (cyoE), partial CSB-YP770075FGP
CSB-EP770075FGP
CSB-BP770075FGP
CSB-MP770075FGP
CSB-EP770075FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP770076FGP
CSB-EP770076FGP
CSB-BP770076FGP
CSB-MP770076FGP
CSB-EP770076FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Alginate biosynthesis protein Alg44 (alg44) CSB-YP770077FGP
CSB-EP770077FGP
CSB-BP770077FGP
CSB-MP770077FGP
CSB-EP770077FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Alginate lyase (algL) CSB-YP770078FGP
CSB-EP770078FGP
CSB-BP770078FGP
CSB-MP770078FGP
CSB-EP770078FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Protein CysZ homolog (cysZ), partial CSB-YP770079FGP1
CSB-EP770079FGP1
CSB-BP770079FGP1
CSB-MP770079FGP1
CSB-EP770079FGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato pseudo-curly top virus Protein C2 (C2, L2) CSB-YP770080TDAC
CSB-EP770080TDAC
CSB-BP770080TDAC
CSB-MP770080TDAC
CSB-EP770080TDAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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