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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Transcription factor bHLH127 (BHLH127) CSB-YP768680DOA
CSB-EP768680DOA
CSB-BP768680DOA
CSB-MP768680DOA
CSB-EP768680DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable D-2-hydroxyglutarate dehydrogenase, mitochondrial (D2HGDH) CSB-YP768681OFG
CSB-EP768681OFG
CSB-BP768681OFG
CSB-MP768681OFG
CSB-EP768681OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Glutaredoxin-C9 (GRXC9) CSB-YP768682OFG
CSB-EP768682OFG
CSB-BP768682OFG
CSB-MP768682OFG
CSB-EP768682OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Growth arrest-specific protein 8 homolog (GAS8) CSB-YP768683DSJ
CSB-EP768683DSJ
CSB-BP768683DSJ
CSB-MP768683DSJ
CSB-EP768683DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana MADS-box transcription factor PHERES 2 (PHE2) CSB-YP768684DOA
CSB-EP768684DOA
CSB-BP768684DOA
CSB-MP768684DOA
CSB-EP768684DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calcium-dependent protein kinase 16 (CPK16), partial CSB-YP768685DOA
CSB-EP768685DOA
CSB-BP768685DOA
CSB-MP768685DOA
CSB-EP768685DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Thioredoxin X, chloroplastic (TRX-X) CSB-YP768686OFG
CSB-EP768686OFG
CSB-BP768686OFG
CSB-MP768686OFG
CSB-EP768686OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable acetolactate synthase 2, chloroplastic (ALS2), partial CSB-YP768687OFG
CSB-EP768687OFG
CSB-BP768687OFG
CSB-MP768687OFG
CSB-EP768687OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable glucuronosyltransferase Os04g0398600 (Os04g0398600, LOC_Os04g32670), partial CSB-YP768688OFG
CSB-EP768688OFG
CSB-BP768688OFG
CSB-MP768688OFG
CSB-EP768688OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative non-inhibitory serpin-Z11 (Os04g0533700, LOC_Os04g45110) CSB-YP768689OFG
CSB-EP768689OFG
CSB-BP768689OFG
CSB-MP768689OFG
CSB-EP768689OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Glutamate receptor 3.1 (GLR3.1), partial CSB-YP768690OFG
CSB-EP768690OFG
CSB-BP768690OFG
CSB-MP768690OFG
CSB-EP768690OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Coproporphyrinogen-III oxidase, chloroplastic (CPX) CSB-YP768691OFG
CSB-EP768691OFG
CSB-BP768691OFG
CSB-MP768691OFG
CSB-EP768691OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Anamorsin homolog 2 (Os04g0682050, LOC_Os04g58564) CSB-YP768692OFG
CSB-EP768692OFG
CSB-BP768692OFG
CSB-MP768692OFG
CSB-EP768692OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable aldo-keto reductase 3 (Os04g0339400, LOC_Os04g27060) CSB-YP768693OFG
CSB-EP768693OFG
CSB-BP768693OFG
CSB-MP768693OFG
CSB-EP768693OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable phytol kinase 1, chloroplastic (Os04g0670700, LOC_Os04g57500), partial CSB-YP768694OFG1
CSB-EP768694OFG1
CSB-BP768694OFG1
CSB-MP768694OFG1
CSB-EP768694OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative inorganic phosphate transporter 1-13 (PHT1-13), partial CSB-YP768695OFG
CSB-EP768695OFG
CSB-BP768695OFG
CSB-MP768695OFG
CSB-EP768695OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cyclin-B2-1 (CYCB2-1) CSB-YP768696OFG
CSB-EP768696OFG
CSB-BP768696OFG
CSB-MP768696OFG
CSB-EP768696OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable aquaporin PIP1-2 (PIP1-2), partial CSB-YP768697OFG1
CSB-EP768697OFG1
CSB-BP768697OFG1
CSB-MP768697OFG1
CSB-EP768697OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable aldo-keto reductase 2 (Os04g0338000, LOC_Os04g26910) CSB-YP768698OFG
CSB-EP768698OFG
CSB-BP768698OFG
CSB-MP768698OFG
CSB-EP768698OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Maltose excess protein 1-like, chloroplastic (Os04g0602400, LOC_Os04g51330), partial CSB-YP768699OFG
CSB-EP768699OFG
CSB-BP768699OFG
CSB-MP768699OFG
CSB-EP768699OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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