重組蛋白
重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實驗室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項目中生成的蛋白質(zhì)。它們在許多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對地方了。
經(jīng)過16多年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達技術(shù),以及膜蛋白表達技術(shù)等。
產(chǎn)品特點
- 超過10,000種重組蛋白覆蓋大多數(shù)基因/靶點;
- 5種表達系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細胞、哺乳動物細胞和體外大腸桿菌。而體外大腸桿菌表達系統(tǒng)是華美生物的獨特表達系統(tǒng)。與傳統(tǒng)蛋白表達系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標準及其原料、體內(nèi)研究、質(zhì)譜標準、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動物副產(chǎn)品的動物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
---|---|---|
Recombinant Human LysM and putative peptidoglycan-binding domain-containing protein 3 (LYSMD3), partial | CSB-YP768760HU CSB-EP768760HU CSB-BP768760HU CSB-MP768760HU CSB-EP768760HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Ankyrin repeat domain-containing protein 33 (ANKRD33) | CSB-YP768761HU CSB-EP768761HU CSB-BP768761HU CSB-MP768761HU CSB-EP768761HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Notch homolog 2 N-terminal-like protein (NOTCH2NL) | CSB-YP768762HU CSB-EP768762HU CSB-BP768762HU CSB-MP768762HU CSB-EP768762HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Keratin, type I cytoskeletal 28 (KRT28) | CSB-YP768763HU CSB-EP768763HU CSB-BP768763HU CSB-MP768763HU CSB-EP768763HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Transmembrane channel-like protein 6 (TMC6), partial | CSB-YP768764HU CSB-EP768764HU CSB-BP768764HU CSB-MP768764HU CSB-EP768764HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human E3 ubiquitin-protein ligase RNF144B (RNF144B), partial | CSB-YP768765HU1 CSB-EP768765HU1 CSB-BP768765HU1 CSB-MP768765HU1 CSB-EP768765HU1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Cytochrome P450 2U1 (CYP2U1), partial | CSB-YP768766HU1 CSB-EP768766HU1 CSB-BP768766HU1 CSB-MP768766HU1 CSB-EP768766HU1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Nephrocystin-3 (NPHP3), partial | CSB-YP768767HU CSB-EP768767HU CSB-BP768767HU CSB-MP768767HU CSB-EP768767HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Cytochrome c oxidase subunit 8C, mitochondrial (COX8C), partial | CSB-YP768768HU CSB-EP768768HU CSB-BP768768HU CSB-MP768768HU CSB-EP768768HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Growth/differentiation factor 7 (GDF7) | CSB-YP768769HU CSB-EP768769HU CSB-BP768769HU CSB-MP768769HU CSB-EP768769HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Hepatoma-derived growth factor-related protein 2 (HDGFRP2) | CSB-YP768770HU CSB-EP768770HU CSB-BP768770HU CSB-MP768770HU CSB-EP768770HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human NANOG neighbor homeobox (NANOGNB) | CSB-YP768771HU CSB-EP768771HU CSB-BP768771HU CSB-MP768771HU CSB-EP768771HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Retinoic acid-induced protein 1 (RAI1), partial | CSB-YP768772HU CSB-EP768772HU CSB-BP768772HU CSB-MP768772HU CSB-EP768772HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Protein sidekick-1 (SDK1), partial | CSB-YP768773HU CSB-EP768773HU CSB-BP768773HU CSB-MP768773HU CSB-EP768773HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Protein phosphatase 1 regulatory subunit 32 (PPP1R32) | CSB-YP768774HU CSB-EP768774HU CSB-BP768774HU CSB-MP768774HU CSB-EP768774HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Neuroepithelial cell-transforming gene 1 protein (NET1) | CSB-YP768775HU CSB-BP768775HU CSB-MP768775HU CSB-EP768775HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human E3 ubiquitin-protein ligase RBBP6 (RBBP6), partial | CSB-YP768776HU CSB-EP768776HU CSB-BP768776HU CSB-MP768776HU CSB-EP768776HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human FYVE, RhoGEF and PH domain-containing protein 2 (FGD2) | CSB-YP768777HU CSB-EP768777HU CSB-BP768777HU CSB-MP768777HU CSB-EP768777HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Transmembrane and coiled-coil domain-containing protein 2 (TMCO2), partial | CSB-YP768778HU1 CSB-EP768778HU1 CSB-BP768778HU1 CSB-MP768778HU1 CSB-EP768778HU1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human HEAT repeat-containing protein 7B2 (HEATR7B2), partial | CSB-YP768779HU CSB-EP768779HU CSB-BP768779HU CSB-MP768779HU CSB-EP768779HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達載體?
表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022