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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio COP9 signalosome complex subunit 8 (cops8) CSB-YP768800DIL
CSB-EP768800DIL
CSB-BP768800DIL
CSB-MP768800DIL
CSB-EP768800DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Malignant T-cell-amplified sequence 1 (mcts1) CSB-YP768801DIL
CSB-EP768801DIL
CSB-BP768801DIL
CSB-MP768801DIL
CSB-EP768801DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Uridine-cytidine kinase 2-B (uck2b) CSB-YP768802DIL
CSB-EP768802DIL
CSB-BP768802DIL
CSB-MP768802DIL
CSB-EP768802DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Aldehyde dehydrogenase family 9 member A1-A (aldh9a1a), partial CSB-YP768803DIL
CSB-EP768803DIL
CSB-BP768803DIL
CSB-MP768803DIL
CSB-EP768803DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Actin, cytoplasmic 2 (actbb) CSB-YP768804DIL
CSB-EP768804DIL
CSB-BP768804DIL
CSB-MP768804DIL
CSB-EP768804DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio WASH complex subunit strumpellin (zgc:55908), partial CSB-YP768805DIL
CSB-EP768805DIL
CSB-BP768805DIL
CSB-MP768805DIL
CSB-EP768805DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cholinephosphotransferase 1 (chpt1), partial CSB-YP768806DIL
CSB-EP768806DIL
CSB-BP768806DIL
CSB-MP768806DIL
CSB-EP768806DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio DNA-directed RNA polymerase II subunit RPB7 (polr2g) CSB-YP768807DIL
CSB-EP768807DIL
CSB-BP768807DIL
CSB-MP768807DIL
CSB-EP768807DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio AP-2 complex subunit mu-B (ap2m1b) CSB-YP768808DIL
CSB-EP768808DIL
CSB-BP768808DIL
CSB-MP768808DIL
CSB-EP768808DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio UMP-CMP kinase (cmpk1) CSB-YP768809DIL
CSB-EP768809DIL
CSB-BP768809DIL
CSB-MP768809DIL
CSB-EP768809DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio 60S ribosomal protein L34 (rpl34) CSB-YP768810DIL
CSB-EP768810DIL
CSB-BP768810DIL
CSB-MP768810DIL
CSB-EP768810DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Midnolin-A (midn-a) CSB-YP768811XBE
CSB-EP768811XBE
CSB-BP768811XBE
CSB-MP768811XBE
CSB-EP768811XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sorting and assembly machinery component 50 homolog A (samm50-a) CSB-YP768812XBE
CSB-EP768812XBE
CSB-BP768812XBE
CSB-MP768812XBE
CSB-EP768812XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis PIH1 domain-containing protein 1 (pih1d1) CSB-YP768813XBE
CSB-EP768813XBE
CSB-BP768813XBE
CSB-MP768813XBE
CSB-EP768813XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase RNF8-A (rnf8-a), partial CSB-YP768814XBE
CSB-EP768814XBE
CSB-BP768814XBE
CSB-MP768814XBE
CSB-EP768814XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cyclin-dependent kinase 9-B (cdk9-b) CSB-YP768815XBE
CSB-EP768815XBE
CSB-BP768815XBE
CSB-MP768815XBE
CSB-EP768815XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nuclear pore complex protein Nup93 (nup93), partial CSB-YP768816XBE
CSB-EP768816XBE
CSB-BP768816XBE
CSB-MP768816XBE
CSB-EP768816XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Proteasomal ubiquitin receptor ADRM1-B (adrm1-b) CSB-YP768817XBE
CSB-EP768817XBE
CSB-BP768817XBE
CSB-MP768817XBE
CSB-EP768817XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NEDD4-binding protein 1 (n4bp1), partial CSB-YP768818XBE
CSB-EP768818XBE
CSB-BP768818XBE
CSB-MP768818XBE
CSB-EP768818XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Signal transducer and activator of transcription 3.2 (stat3.2), partial CSB-YP768819XBE
CSB-EP768819XBE
CSB-BP768819XBE
CSB-MP768819XBE
CSB-EP768819XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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