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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human HEAT repeat-containing protein 5A (HEATR5A), partial CSB-YP769801HU
CSB-EP769801HU
CSB-BP769801HU
CSB-MP769801HU
CSB-EP769801HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein phosphatase 1 regulatory subunit 3B (PPP1R3B) CSB-YP769802HU
CSB-EP769802HU
CSB-BP769802HU
CSB-MP769802HU
CSB-EP769802HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human V-set and immunoglobulin domain-containing protein 1 (VSIG1), partial CSB-YP769803HU
CSB-EP769803HU
CSB-BP769803HU
CSB-MP769803HU
CSB-EP769803HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Diacylglycerol kinase eta (DGKH), partial CSB-YP769804HU
CSB-EP769804HU
CSB-BP769804HU
CSB-MP769804HU
CSB-EP769804HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Centrosomal protein of 57 kDa (CEP57) CSB-YP769805HU
CSB-EP769805HU
CSB-BP769805HU
CSB-MP769805HU
CSB-EP769805HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Extracellular matrix protein FRAS1 (FRAS1), partial CSB-YP769806HU
CSB-EP769806HU
CSB-BP769806HU
CSB-MP769806HU
CSB-EP769806HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Probable G-protein coupled receptor 97 (GPR97), partial CSB-YP769807HU1
CSB-EP769807HU1
CSB-BP769807HU1
CSB-MP769807HU1
CSB-EP769807HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Histone-lysine N-methyltransferase SUV420H2 (SUV420H2) CSB-YP769808HU
CSB-EP769808HU
CSB-BP769808HU
CSB-MP769808HU
CSB-EP769808HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Prostate tumor-overexpressed gene 1 protein (PTOV1) CSB-YP769809HU
CSB-EP769809HU
CSB-BP769809HU
CSB-MP769809HU
CSB-EP769809HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 5'-nucleotidase domain-containing protein 4 (NT5DC4) CSB-YP769810HU
CSB-EP769810HU
CSB-BP769810HU
CSB-MP769810HU
CSB-EP769810HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human E3 ubiquitin-protein ligase MARCH9 (41342), partial CSB-YP769811HU1
CSB-EP769811HU1
CSB-BP769811HU1
CSB-MP769811HU1
CSB-EP769811HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Peroxisome proliferator-activated receptor gamma coactivator 1-beta (PPARGC1B), partial CSB-YP769812HU
CSB-BP769812HU
CSB-MP769812HU
CSB-EP769812HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein KIAA0528 (KIAA0528), partial CSB-YP769813HU
CSB-EP769813HU
CSB-BP769813HU
CSB-MP769813HU
CSB-EP769813HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Myosin light chain kinase family member 4 (MYLK4) CSB-YP769814HU
CSB-EP769814HU
CSB-BP769814HU
CSB-MP769814HU
CSB-EP769814HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Beta-klotho (KLB), partial CSB-YP769815HU
CSB-EP769815HU
CSB-BP769815HU
CSB-MP769815HU
CSB-EP769815HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Catabolic 3-dehydroquinase (qutE) CSB-YP769816EXJ
CSB-EP769816EXJ
CSB-BP769816EXJ
CSB-MP769816EXJ
CSB-EP769816EXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Subterranean clover stunt virus Putative movement protein (DNA-M), partial CSB-YP769817SHAO1
CSB-EP769817SHAO1
CSB-BP769817SHAO1
CSB-MP769817SHAO1
CSB-EP769817SHAO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae virus L-BC Major capsid protein (gag), partial CSB-YP769818SAF
CSB-EP769818SAF
CSB-BP769818SAF
CSB-MP769818SAF
CSB-EP769818SAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suid herpesvirus 1 Glycoprotein N (GN), partial CSB-YP769819SRI1
CSB-EP769819SRI1
CSB-BP769819SRI1
CSB-MP769819SRI1
CSB-EP769819SRI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YOL013W-B (YOL013W-B), partial CSB-YP769820SVG
CSB-EP769820SVG
CSB-BP769820SVG
CSB-MP769820SVG
CSB-EP769820SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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