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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis N-alpha-acetyltransferase 20 (naa20) CSB-YP768820XBE
CSB-EP768820XBE
CSB-BP768820XBE
CSB-MP768820XBE
CSB-EP768820XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis N-lysine methyltransferase SMYD2-A (smyd2-a) CSB-YP768821XBE
CSB-EP768821XBE
CSB-BP768821XBE
CSB-MP768821XBE
CSB-EP768821XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis F-box/SPRY domain-containing protein 1 (fbxo45) CSB-YP768822XBE
CSB-EP768822XBE
CSB-BP768822XBE
CSB-MP768822XBE
CSB-EP768822XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Homeobox protein meis3-B (meis3-b) CSB-YP768823XBE
CSB-EP768823XBE
CSB-BP768823XBE
CSB-MP768823XBE
CSB-EP768823XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis HEAT repeat-containing protein 6 (heatr6), partial CSB-YP768824XBE
CSB-EP768824XBE
CSB-BP768824XBE
CSB-MP768824XBE
CSB-EP768824XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nucleosome assembly protein 1-like 1-B (nap1l1-b) CSB-YP768825XBE
CSB-EP768825XBE
CSB-BP768825XBE
CSB-MP768825XBE
CSB-EP768825XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Polyadenylate-binding protein-interacting protein 1 (paip1) CSB-YP768826XBE
CSB-EP768826XBE
CSB-BP768826XBE
CSB-MP768826XBE
CSB-EP768826XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Target of rapamycin complex 2 subunit MAPKAP1 (mapkap1), partial CSB-YP768827XBE
CSB-EP768827XBE
CSB-BP768827XBE
CSB-MP768827XBE
CSB-EP768827XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DDB1- and CUL4-associated factor 13 (dcaf13) CSB-YP768828XBE
CSB-EP768828XBE
CSB-BP768828XBE
CSB-MP768828XBE
CSB-EP768828XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio AKT-interacting protein (aktip) CSB-YP768829DIL
CSB-EP768829DIL
CSB-BP768829DIL
CSB-MP768829DIL
CSB-EP768829DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serine/threonine-protein kinase Nek9 (nek9), partial CSB-YP768830XBE
CSB-EP768830XBE
CSB-BP768830XBE
CSB-MP768830XBE
CSB-EP768830XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Deleted in bladder cancer protein 1 homolog (DBC1), partial CSB-YP768831CH
CSB-EP768831CH
CSB-BP768831CH
CSB-MP768831CH
CSB-EP768831CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Heparan-sulfate 6-O-sulfotransferase 2 (hs6st2), partial CSB-YP768832DIL
CSB-EP768832DIL
CSB-BP768832DIL
CSB-MP768832DIL
CSB-EP768832DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Transcription factor 7-like 1-B (tcf7l1b), partial CSB-YP768833DIL
CSB-EP768833DIL
CSB-BP768833DIL
CSB-MP768833DIL
CSB-EP768833DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis MIF4G domain-containing protein B (mif4gd-b) CSB-YP768834XBE
CSB-EP768834XBE
CSB-BP768834XBE
CSB-MP768834XBE
CSB-EP768834XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Glycerol-3-phosphate dehydrogenase 1-like protein (gpd1l) CSB-YP768835XBE
CSB-EP768835XBE
CSB-BP768835XBE
CSB-MP768835XBE
CSB-EP768835XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja sputatrix Weak neurotoxin 8 (WNTX8) CSB-YP768836NFH
CSB-EP768836NFH
CSB-BP768836NFH
CSB-MP768836NFH
CSB-EP768836NFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio 15 kDa selenoprotein (41532) CSB-YP768837DIL
CSB-EP768837DIL
CSB-BP768837DIL
CSB-MP768837DIL
CSB-EP768837DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Abhydrolase domain-containing protein 2-A (abhd2a), partial CSB-YP768838DIL1
CSB-EP768838DIL1
CSB-BP768838DIL1
CSB-MP768838DIL1
CSB-EP768838DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase RNF8 (rnf8) CSB-YP768839DIL
CSB-EP768839DIL
CSB-BP768839DIL
CSB-MP768839DIL
CSB-EP768839DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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