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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Dictyostelium discoideum Membrane magnesium transporter homolog (mmgt), partial CSB-YP769741DKK
CSB-EP769741DKK
CSB-BP769741DKK
CSB-MP769741DKK
CSB-EP769741DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Signal peptidase complex catalytic subunit sec11 (sec11), partial CSB-YP769742DKK
CSB-EP769742DKK
CSB-BP769742DKK
CSB-MP769742DKK
CSB-EP769742DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative transmembrane protein DDB_G0272126 (DDB_G0272126), partial CSB-YP769743DKK1
CSB-EP769743DKK1
CSB-BP769743DKK1
CSB-MP769743DKK1
CSB-EP769743DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum UPF0548 protein (DDB_G0271742) CSB-YP769744DKK
CSB-EP769744DKK
CSB-BP769744DKK
CSB-MP769744DKK
CSB-EP769744DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ras-related protein RabR (rabR) CSB-YP769745DKK
CSB-EP769745DKK
CSB-BP769745DKK
CSB-MP769745DKK
CSB-EP769745DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum TNF receptor-associated factor family protein DDB_G0277243 (DDB_G0277243) CSB-YP769746DKK
CSB-EP769746DKK
CSB-BP769746DKK
CSB-MP769746DKK
CSB-EP769746DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0277209 (DDB_G0277209) CSB-YP769747DKK
CSB-EP769747DKK
CSB-BP769747DKK
CSB-MP769747DKK
CSB-EP769747DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0274171 (DDB_G0274171) CSB-YP769748DKK
CSB-EP769748DKK
CSB-BP769748DKK
CSB-MP769748DKK
CSB-EP769748DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0272516 (DDB_G0272516) CSB-YP769749DKK
CSB-EP769749DKK
CSB-BP769749DKK
CSB-MP769749DKK
CSB-EP769749DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Membrane selenoprotein (msp), partial CSB-YP769750DKK1
CSB-EP769750DKK1
CSB-BP769750DKK1
CSB-MP769750DKK1
CSB-EP769750DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum TNF receptor-associated protein 1 homolog, mitochondrial (trap1), partial CSB-YP769751DKK
CSB-EP769751DKK
CSB-BP769751DKK
CSB-MP769751DKK
CSB-EP769751DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ras-related protein rapB (rapB) CSB-YP769752DKK
CSB-EP769752DKK
CSB-BP769752DKK
CSB-MP769752DKK
CSB-EP769752DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesobuthus martensii Neurotoxin BmKAEP2 CSB-YP769753MRE
CSB-EP769753MRE
CSB-BP769753MRE
CSB-MP769753MRE
CSB-EP769753MRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rho GTPase-activating protein syd-1 (syd-1), partial CSB-YP769754CXY
CSB-EP769754CXY
CSB-BP769754CXY
CSB-MP769754CXY
CSB-EP769754CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex pipiens pipiens Cecropin-B2 (CECB2) CSB-YP769755CUL
CSB-EP769755CUL
CSB-BP769755CUL
CSB-MP769755CUL
CSB-EP769755CUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dermacentor variabilis Defensin (VSNA1) CSB-YP769756DCAV
CSB-EP769756DCAV
CSB-BP769756DCAV
CSB-MP769756DCAV
CSB-EP769756DCAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides exilicauda Potassium channel toxin gamma-KTx 4.4 (ERG3) CSB-YP769757CFAY
CSB-EP769757CFAY
CSB-BP769757CFAY
CSB-MP769757CFAY
CSB-EP769757CFAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides sculpturatus Potassium channel toxin gamma-KTx 4.9 (ERG3) CSB-YP769758CGD
CSB-EP769758CGD
CSB-BP769758CGD
CSB-MP769758CGD
CSB-EP769758CGD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides limpidus limpidus Potassium channel toxin gamma-KTx 4.1 (ERG2) CSB-YP769759CGA
CSB-EP769759CGA
CSB-BP769759CGA
CSB-MP769759CGA
CSB-EP769759CGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides elegans Potassium channel toxin gamma-KTx 1.2 (ERG1) CSB-YP769760CFW
CSB-EP769760CFW
CSB-BP769760CFW
CSB-MP769760CFW
CSB-EP769760CFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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