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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neurospora crassa Transcription factor iws-1 (iws-1) CSB-YP769821NHA
CSB-EP769821NHA
CSB-BP769821NHA
CSB-MP769821NHA
CSB-EP769821NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Cytochrome b-c1 complex subunit 7 (qcr-7) CSB-YP769822NHA
CSB-EP769822NHA
CSB-BP769822NHA
CSB-MP769822NHA
CSB-EP769822NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Vacuolar-sorting protein snf-7 (snf-7) CSB-YP769823NHA
CSB-EP769823NHA
CSB-BP769823NHA
CSB-MP769823NHA
CSB-EP769823NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa ATP-dependent rRNA helicase spb-4 (spb-4), partial CSB-YP769824NHA
CSB-EP769824NHA
CSB-BP769824NHA
CSB-MP769824NHA
CSB-EP769824NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gibberella moniliformis pH-response transcription factor pacC/RIM101 (PAC1), partial CSB-YP769825GAAU
CSB-EP769825GAAU
CSB-BP769825GAAU
CSB-MP769825GAAU
CSB-EP769825GAAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces delphensis Probable endonuclease LCL3 (LCL3), partial CSB-YP769826KAAF1
CSB-EP769826KAAF1
CSB-BP769826KAAF1
CSB-MP769826KAAF1
CSB-EP769826KAAF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe GTP-binding protein rho4 (rho4) CSB-YP769827SXV
CSB-EP769827SXV
CSB-BP769827SXV
CSB-MP769827SXV
CSB-EP769827SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Oxidation resistance protein 1 (OXR1) CSB-YP769828EXI
CSB-EP769828EXI
CSB-BP769828EXI
CSB-MP769828EXI
CSB-EP769828EXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Altered inheritance of mitochondria protein 31, mitochondrial (AIM31), partial CSB-YP769829EXJ1
CSB-EP769829EXJ1
CSB-BP769829EXJ1
CSB-MP769829EXJ1
CSB-EP769829EXJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hypocrea virens Tubulin beta-2 chain CSB-YP769830HYF
CSB-EP769830HYF
CSB-BP769830HYF
CSB-MP769830HYF
CSB-EP769830HYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea kluyveri Protein transport protein SEC24 (SEC24), partial CSB-YP769831LMA
CSB-EP769831LMA
CSB-BP769831LMA
CSB-MP769831LMA
CSB-EP769831LMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naumovozyma castellii Protein transport protein SEC24-1 (SEC241), partial CSB-YP769832NAY
CSB-EP769832NAY
CSB-BP769832NAY
CSB-MP769832NAY
CSB-EP769832NAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces bayanus Myosin-2 (MYO2), partial CSB-YP769833SVF
CSB-EP769833SVF
CSB-BP769833SVF
CSB-MP769833SVF
CSB-EP769833SVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hypocrea jecorina NAD (P)H-dependent D-xylose reductase xyl1 (xyl1) CSB-YP769834HYE
CSB-EP769834HYE
CSB-BP769834HYE
CSB-MP769834HYE
CSB-EP769834HYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus oryzae Small COPII coat GTPase sar1 (sar1) CSB-YP769835DPA
CSB-EP769835DPA
CSB-BP769835DPA
CSB-MP769835DPA
CSB-EP769835DPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tufA) CSB-YP769836VFE
CSB-EP769836VFE
CSB-BP769836VFE
CSB-MP769836VFE
CSB-EP769836VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP769837XAT
CSB-EP769837XAT
CSB-BP769837XAT
CSB-MP769837XAT
CSB-EP769837XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 50S ribosomal protein L10 (rplJ) CSB-YP769838XAT
CSB-EP769838XAT
CSB-BP769838XAT
CSB-MP769838XAT
CSB-EP769838XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Putative pterin-4-alpha-carbinolamine dehydratase (PD_1980) CSB-YP769839XAT
CSB-EP769839XAT
CSB-BP769839XAT
CSB-MP769839XAT
CSB-EP769839XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP769840XAT
CSB-EP769840XAT
CSB-BP769840XAT
CSB-MP769840XAT
CSB-EP769840XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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