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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mesobuthus martensii Makatoxin-2 (MKTXII) CSB-YP769721MRE
CSB-EP769721MRE
CSB-BP769721MRE
CSB-MP769721MRE
CSB-EP769721MRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Soluble guanylate cyclase gcy-31 (gcy-31), partial CSB-YP769722CXY
CSB-EP769722CXY
CSB-BP769722CXY
CSB-MP769722CXY
CSB-EP769722CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma belcheri 40S ribosomal protein S21 (RPS21) CSB-YP769723BVX
CSB-EP769723BVX
CSB-BP769723BVX
CSB-MP769723BVX
CSB-EP769723BVX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dermacentor variabilis 40S ribosomal protein S23 (RpS23) CSB-YP769724DCAV
CSB-EP769724DCAV
CSB-BP769724DCAV
CSB-MP769724DCAV
CSB-EP769724DCAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex torrentium Acetylcholinesterase (ACE-1) CSB-YP769725CIAV
CSB-EP769725CIAV
CSB-BP769725CIAV
CSB-MP769725CIAV
CSB-EP769725CIAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Proline dehydrogenase 1, mitochondrial (prodh), partial CSB-YP769726DKK
CSB-EP769726DKK
CSB-BP769726DKK
CSB-MP769726DKK
CSB-EP769726DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Protein ACN9 homolog, mitochondrial (acn9), partial CSB-YP769727DKK
CSB-EP769727DKK
CSB-BP769727DKK
CSB-MP769727DKK
CSB-EP769727DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum HssA/B-like protein 24 (hssl24) CSB-YP769728DKK
CSB-EP769728DKK
CSB-BP769728DKK
CSB-MP769728DKK
CSB-EP769728DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Molybdopterin synthase catalytic subunit (mocs2l) CSB-YP769729DKK
CSB-EP769729DKK
CSB-BP769729DKK
CSB-MP769729DKK
CSB-EP769729DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Mitochondrial substrate carrier family protein Y (mcfY), partial CSB-YP769730DKK1
CSB-EP769730DKK1
CSB-BP769730DKK1
CSB-MP769730DKK1
CSB-EP769730DKK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Probable ubiquinone biosynthesis protein coq9, mitochondrial (coq9), partial CSB-YP769731DKK
CSB-EP769731DKK
CSB-BP769731DKK
CSB-MP769731DKK
CSB-EP769731DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Oxysterol-binding protein 6 (osbF) CSB-YP769732DKK
CSB-EP769732DKK
CSB-BP769732DKK
CSB-MP769732DKK
CSB-EP769732DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Myb-like protein K (mybK), partial CSB-YP769733DKK
CSB-EP769733DKK
CSB-BP769733DKK
CSB-MP769733DKK
CSB-EP769733DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Uncharacterized protein DDB_G0275275 (DDB_G0275275), partial CSB-YP769734DKK
CSB-EP769734DKK
CSB-BP769734DKK
CSB-MP769734DKK
CSB-EP769734DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0271896 (DDB_G0271896) CSB-YP769735DKK
CSB-EP769735DKK
CSB-BP769735DKK
CSB-MP769735DKK
CSB-EP769735DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative uncharacterized protein DDB_G0275639 (DDB_G0275639) CSB-YP769736DKK
CSB-EP769736DKK
CSB-BP769736DKK
CSB-MP769736DKK
CSB-EP769736DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Rho GTPase-activating protein gacH (gacH), partial CSB-YP769737DKK
CSB-EP769737DKK
CSB-BP769737DKK
CSB-MP769737DKK
CSB-EP769737DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Serine/threonine-protein phosphatase 4 regulatory subunit 2 (ppp4r2) CSB-YP769738DKK
CSB-EP769738DKK
CSB-BP769738DKK
CSB-MP769738DKK
CSB-EP769738DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Calcium-binding protein K-like (DDB_G0274357) CSB-YP769739DKK
CSB-EP769739DKK
CSB-BP769739DKK
CSB-MP769739DKK
CSB-EP769739DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cofilin-5 (cofF) CSB-YP769740DKK
CSB-EP769740DKK
CSB-BP769740DKK
CSB-MP769740DKK
CSB-EP769740DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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