Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas syringae pv. tomato Coenzyme PQQ synthesis protein A (pqqA) CSB-YP771758FGP
CSB-EP771758FGP
CSB-BP771758FGP
CSB-MP771758FGP
CSB-EP771758FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Coenzyme PQQ synthesis protein E (pqqE) CSB-YP771759FGP
CSB-EP771759FGP
CSB-BP771759FGP
CSB-MP771759FGP
CSB-EP771759FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Betaine aldehyde dehydrogenase (betB) CSB-YP771760FGP
CSB-EP771760FGP
CSB-BP771760FGP
CSB-MP771760FGP
CSB-EP771760FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Phosphoglycerate kinase (pgk) CSB-YP771761FGP
CSB-EP771761FGP
CSB-BP771761FGP
CSB-MP771761FGP
CSB-EP771761FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Alginate biosynthesis transcriptional regulatory protein AlgB (algB) CSB-YP771762FGP
CSB-EP771762FGP
CSB-BP771762FGP
CSB-MP771762FGP
CSB-EP771762FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Ribosomal protein S6 modification protein (rimK) CSB-YP771763FGP
CSB-EP771763FGP
CSB-BP771763FGP
CSB-MP771763FGP
CSB-EP771763FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP771764FGP
CSB-EP771764FGP
CSB-BP771764FGP
CSB-MP771764FGP
CSB-EP771764FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Tryptophan synthase alpha chain (trpA) CSB-YP771765FGP
CSB-EP771765FGP
CSB-BP771765FGP
CSB-MP771765FGP
CSB-EP771765FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Xanthine phosphoribosyltransferase (xpt) CSB-YP771766FGP
CSB-EP771766FGP
CSB-BP771766FGP
CSB-MP771766FGP
CSB-EP771766FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Guanylate kinase (gmk) CSB-YP771767FGP
CSB-EP771767FGP
CSB-BP771767FGP
CSB-MP771767FGP
CSB-EP771767FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP synthase subunit alpha (atpA), partial CSB-YP771768FGB
CSB-EP771768FGB
CSB-BP771768FGB
CSB-MP771768FGB
CSB-EP771768FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Cardiolipin synthase (cls), partial CSB-YP771769FGB1
CSB-EP771769FGB1
CSB-BP771769FGB1
CSB-MP771769FGB1
CSB-EP771769FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Phosphomannomutase/phosphoglucomutase (algC) CSB-YP771770FGB
CSB-EP771770FGB
CSB-BP771770FGB
CSB-MP771770FGB
CSB-EP771770FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 50S ribosomal protein L33 (rpmG) CSB-YP771771FGB
CSB-EP771771FGB
CSB-BP771771FGB
CSB-MP771771FGB
CSB-EP771771FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0149 protein PP_5201 (PP_5201) CSB-YP771772FGB
CSB-EP771772FGB
CSB-BP771772FGB
CSB-MP771772FGB
CSB-EP771772FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Sulfate/thiosulfate import ATP-binding protein CysA (cysA) CSB-YP771773FGB
CSB-EP771773FGB
CSB-BP771773FGB
CSB-MP771773FGB
CSB-EP771773FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Dihydroxy-acid dehydratase (ilvD), partial CSB-YP771774FGB
CSB-EP771774FGB
CSB-BP771774FGB
CSB-MP771774FGB
CSB-EP771774FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Non-canonical purine NTP pyrophosphatase (PP_5100) CSB-YP771775FGB
CSB-EP771775FGB
CSB-BP771775FGB
CSB-MP771775FGB
CSB-EP771775FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 3-dehydroquinate synthase (aroB) CSB-YP771776FGB
CSB-EP771776FGB
CSB-BP771776FGB
CSB-MP771776FGB
CSB-EP771776FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP771777FGB
CSB-EP771777FGB
CSB-BP771777FGB
CSB-MP771777FGB
CSB-EP771777FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>