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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas syringae pv. tomato Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP770041FGP
CSB-EP770041FGP
CSB-BP770041FGP
CSB-MP770041FGP
CSB-EP770041FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP770042FGP
CSB-EP770042FGP
CSB-BP770042FGP
CSB-MP770042FGP
CSB-EP770042FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato UPF0133 protein PSPTO_3645 (PSPTO_3645) CSB-YP770043FGP
CSB-EP770043FGP
CSB-BP770043FGP
CSB-MP770043FGP
CSB-EP770043FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Carbon storage regulator homolog 3 (csrA3) CSB-YP770044FGP
CSB-EP770044FGP
CSB-BP770044FGP
CSB-MP770044FGP
CSB-EP770044FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Probable S-methyl-5'-thioinosine phosphorylase (PSPTO_3506) CSB-YP770045FGP
CSB-EP770045FGP
CSB-BP770045FGP
CSB-MP770045FGP
CSB-EP770045FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP770046FGP
CSB-EP770046FGP
CSB-BP770046FGP
CSB-MP770046FGP
CSB-EP770046FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato DNA translocase FtsK (ftsK), partial CSB-YP770047FGP
CSB-EP770047FGP
CSB-BP770047FGP
CSB-MP770047FGP
CSB-EP770047FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Probable transcriptional regulatory protein PSPTO_3162 (PSPTO_3162) CSB-YP770048FGP
CSB-EP770048FGP
CSB-BP770048FGP
CSB-MP770048FGP
CSB-EP770048FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Xylose import ATP-binding protein XylG (xylG), partial CSB-YP770049FGP
CSB-EP770049FGP
CSB-BP770049FGP
CSB-MP770049FGP
CSB-EP770049FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Aliphatic sulfonates import ATP-binding protein SsuB 2 (ssuB2) CSB-YP770050FGP
CSB-EP770050FGP
CSB-BP770050FGP
CSB-MP770050FGP
CSB-EP770050FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP770051FGP
CSB-EP770051FGP
CSB-BP770051FGP
CSB-MP770051FGP
CSB-EP770051FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Satellite St. Augustine decline virus Coat protein CSB-YP770052SHAR
CSB-EP770052SHAR
CSB-BP770052SHAR
CSB-MP770052SHAR
CSB-EP770052SHAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Urease accessory protein UreF 1 (ureF1) CSB-YP770053FGP
CSB-EP770053FGP
CSB-BP770053FGP
CSB-MP770053FGP
CSB-EP770053FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Threonine--tRNA ligase (thrS), partial CSB-YP770054FGP
CSB-EP770054FGP
CSB-BP770054FGP
CSB-MP770054FGP
CSB-EP770054FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato Potassium-transporting ATPase C chain (kdpC), partial CSB-YP770055FGP1
CSB-EP770055FGP1
CSB-BP770055FGP1
CSB-MP770055FGP1
CSB-EP770055FGP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus SpV1-C74 Uncharacterized protein ORF5 (ORF5), partial CSB-YP770056SGAH1
CSB-EP770056SGAH1
CSB-BP770056SGAH1
CSB-MP770056SGAH1
CSB-EP770056SGAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spiroplasma virus SpV1-C74 Uncharacterized protein ORF10 (ORF10), partial CSB-YP770057SGAH1
CSB-EP770057SGAH1
CSB-BP770057SGAH1
CSB-MP770057SGAH1
CSB-EP770057SGAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tacaribe virus RING finger protein Z (Z) CSB-YP770058TAC
CSB-EP770058TAC
CSB-BP770058TAC
CSB-MP770058TAC
CSB-EP770058TAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato tRNA-dihydrouridine synthase C (dusC) CSB-YP770059FGP
CSB-EP770059FGP
CSB-BP770059FGP
CSB-MP770059FGP
CSB-EP770059FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas syringae pv. tomato NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP770060FGP
CSB-EP770060FGP
CSB-BP770060FGP
CSB-MP770060FGP
CSB-EP770060FGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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