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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas putida Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP770121FGB
CSB-EP770121FGB
CSB-BP770121FGB
CSB-MP770121FGB
CSB-EP770121FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Thiamine-phosphate synthase (thiE) CSB-YP770122FGB
CSB-EP770122FGB
CSB-BP770122FGB
CSB-MP770122FGB
CSB-EP770122FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Dihydrodipicolinate reductase (dapB) CSB-YP770123FGB
CSB-EP770123FGB
CSB-BP770123FGB
CSB-MP770123FGB
CSB-EP770123FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 30S ribosomal protein S15 (rpsO) CSB-YP770124FGB
CSB-EP770124FGB
CSB-BP770124FGB
CSB-MP770124FGB
CSB-EP770124FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Sugar fermentation stimulation protein homolog (sfsA) CSB-YP770125FGB
CSB-EP770125FGB
CSB-BP770125FGB
CSB-MP770125FGB
CSB-EP770125FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Homogentisate 1,2-dioxygenase (hmgA) CSB-YP770126FGB
CSB-EP770126FGB
CSB-BP770126FGB
CSB-MP770126FGB
CSB-EP770126FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Pterin-4-alpha-carbinolamine dehydratase (phhB) CSB-YP770127FGB
CSB-EP770127FGB
CSB-BP770127FGB
CSB-MP770127FGB
CSB-EP770127FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida D-amino acid dehydrogenase 1 small subunit (dadA1) CSB-YP770128FGB
CSB-EP770128FGB
CSB-BP770128FGB
CSB-MP770128FGB
CSB-EP770128FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida D-alanine--D-alanine ligase A (ddlA) CSB-YP770129FGB
CSB-EP770129FGB
CSB-BP770129FGB
CSB-MP770129FGB
CSB-EP770129FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA ligase (ligA), partial CSB-YP770130FGB
CSB-EP770130FGB
CSB-BP770130FGB
CSB-MP770130FGB
CSB-EP770130FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP770131FGB
CSB-EP770131FGB
CSB-BP770131FGB
CSB-MP770131FGB
CSB-EP770131FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP770132FGB
CSB-EP770132FGB
CSB-BP770132FGB
CSB-MP770132FGB
CSB-EP770132FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP770133FGB1
CSB-EP770133FGB1
CSB-BP770133FGB1
CSB-MP770133FGB1
CSB-EP770133FGB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP770134FGB
CSB-EP770134FGB
CSB-BP770134FGB
CSB-MP770134FGB
CSB-EP770134FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Outer-membrane lipoprotein carrier protein (lolA) CSB-YP770135FGB
CSB-EP770135FGB
CSB-BP770135FGB
CSB-MP770135FGB
CSB-EP770135FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Nickel import ATP-binding protein NikD (nikD) CSB-YP770136FGB
CSB-EP770136FGB
CSB-BP770136FGB
CSB-MP770136FGB
CSB-EP770136FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ecotin (eco) CSB-YP770137FGB
CSB-EP770137FGB
CSB-BP770137FGB
CSB-MP770137FGB
CSB-EP770137FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glutamine--tRNA ligase (glnS), partial CSB-YP770138FGB
CSB-EP770138FGB
CSB-BP770138FGB
CSB-MP770138FGB
CSB-EP770138FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Urease accessory protein UreF (ureF) CSB-YP770139FGB
CSB-EP770139FGB
CSB-BP770139FGB
CSB-MP770139FGB
CSB-EP770139FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Ribose import ATP-binding protein RbsA (rbsA), partial CSB-YP770140FGB
CSB-EP770140FGB
CSB-BP770140FGB
CSB-MP770140FGB
CSB-EP770140FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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