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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chlamydophila caviae Phosphoglycerate kinase (pgk) CSB-YP772432DSL
CSB-EP772432DSL
CSB-BP772432DSL
CSB-MP772432DSL
CSB-EP772432DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydophila caviae Probable endonuclease 4 (nfo) CSB-YP772433DSL
CSB-EP772433DSL
CSB-BP772433DSL
CSB-MP772433DSL
CSB-EP772433DSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP772434IDP
CSB-EP772434IDP
CSB-BP772434IDP
CSB-MP772434IDP
CSB-EP772434IDP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bursal disease virus Protein VP5 (VP5), partial CSB-YP772435AGAO
CSB-EP772435AGAO
CSB-BP772435AGAO
CSB-MP772435AGAO
CSB-EP772435AGAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Infectious hematopoietic necrosis virus Glycoprotein (G), partial CSB-YP772436ICAJ1
CSB-EP772436ICAJ1
CSB-BP772436ICAJ1
CSB-MP772436ICAJ1
CSB-EP772436ICAJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein grpE 2 (grpE2) CSB-YP772437SNN
CSB-EP772437SNN
CSB-BP772437SNN
CSB-MP772437SNN
CSB-EP772437SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent Clp protease proteolytic subunit 5 (clpP5) CSB-YP772438SNN
CSB-EP772438SNN
CSB-BP772438SNN
CSB-MP772438SNN
CSB-EP772438SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase 2 (uppP2), partial CSB-YP772439SNN1
CSB-EP772439SNN1
CSB-BP772439SNN1
CSB-MP772439SNN1
CSB-EP772439SNN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP772440SNN
CSB-EP772440SNN
CSB-BP772440SNN
CSB-MP772440SNN
CSB-EP772440SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartate carbamoyltransferase (pyrB) CSB-YP772441SNN
CSB-EP772441SNN
CSB-BP772441SNN
CSB-MP772441SNN
CSB-EP772441SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chorismate synthase (aroC) CSB-YP772442SNN
CSB-EP772442SNN
CSB-BP772442SNN
CSB-MP772442SNN
CSB-EP772442SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Argininosuccinate synthase (argG) CSB-YP772443SNN
CSB-EP772443SNN
CSB-BP772443SNN
CSB-MP772443SNN
CSB-EP772443SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetylglutamate kinase (argB) CSB-YP772444SNN
CSB-EP772444SNN
CSB-BP772444SNN
CSB-MP772444SNN
CSB-EP772444SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L20 (rplT) CSB-YP772445SNN
CSB-EP772445SNN
CSB-BP772445SNN
CSB-MP772445SNN
CSB-EP772445SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prokaryotic ubiquitin-like protein Pup (pup) CSB-YP772446SNN
CSB-EP772446SNN
CSB-BP772446SNN
CSB-MP772446SNN
CSB-EP772446SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase Der (der) CSB-YP772447SNN
CSB-EP772447SNN
CSB-BP772447SNN
CSB-MP772447SNN
CSB-EP772447SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative 3-methyladenine DNA glycosylase (SAV_6486) CSB-YP772448SNN
CSB-EP772448SNN
CSB-BP772448SNN
CSB-MP772448SNN
CSB-EP772448SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-ectoine synthase (ectC) CSB-YP772449SNN
CSB-EP772449SNN
CSB-BP772449SNN
CSB-MP772449SNN
CSB-EP772449SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glucose-6-phosphate isomerase 2 (pgi2), partial CSB-YP772450SNN
CSB-EP772450SNN
CSB-BP772450SNN
CSB-MP772450SNN
CSB-EP772450SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UvrABC system protein A (uvrA), partial CSB-YP772451SNN
CSB-EP772451SNN
CSB-BP772451SNN
CSB-MP772451SNN
CSB-EP772451SNN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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