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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas putida Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP771818FGB
CSB-EP771818FGB
CSB-BP771818FGB
CSB-MP771818FGB
CSB-EP771818FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP771819FGB
CSB-EP771819FGB
CSB-BP771819FGB
CSB-MP771819FGB
CSB-EP771819FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Lipid-A-disaccharide synthase (lpxB) CSB-YP771820FGB
CSB-EP771820FGB
CSB-BP771820FGB
CSB-MP771820FGB
CSB-EP771820FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Undecaprenyl pyrophosphate synthase (uppS) CSB-YP771821FGB
CSB-EP771821FGB
CSB-BP771821FGB
CSB-MP771821FGB
CSB-EP771821FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP771822FGB
CSB-EP771822FGB
CSB-BP771822FGB
CSB-MP771822FGB
CSB-EP771822FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Chemotaxis response regulator protein-glutamate methylesterase of group 3 operon (cheB3) CSB-YP771823FGB
CSB-EP771823FGB
CSB-BP771823FGB
CSB-MP771823FGB
CSB-EP771823FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP771824FGB
CSB-EP771824FGB
CSB-BP771824FGB
CSB-MP771824FGB
CSB-EP771824FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida 60 kDa chaperonin (groL), partial CSB-YP771825FGB
CSB-EP771825FGB
CSB-BP771825FGB
CSB-MP771825FGB
CSB-EP771825FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP771826FGB
CSB-EP771826FGB
CSB-BP771826FGB
CSB-MP771826FGB
CSB-EP771826FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0102 protein PP_1324 (PP_1324) CSB-YP771827FGB
CSB-EP771827FGB
CSB-BP771827FGB
CSB-MP771827FGB
CSB-EP771827FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida GDP-mannose 6-dehydrogenase (algD) CSB-YP771828FGB
CSB-EP771828FGB
CSB-BP771828FGB
CSB-MP771828FGB
CSB-EP771828FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Poly (beta-D-mannuronate) C5 epimerase (algG) CSB-YP771829FGB
CSB-EP771829FGB
CSB-BP771829FGB
CSB-MP771829FGB
CSB-EP771829FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Probable malate:quinone oxidoreductase 2 (mqo2), partial CSB-YP771830FGB
CSB-EP771830FGB
CSB-BP771830FGB
CSB-MP771830FGB
CSB-EP771830FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Proline--tRNA ligase (proS), partial CSB-YP771831FGB
CSB-EP771831FGB
CSB-BP771831FGB
CSB-MP771831FGB
CSB-EP771831FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0225 protein PP_1119 (PP_1119) CSB-YP771832FGB
CSB-EP771832FGB
CSB-BP771832FGB
CSB-MP771832FGB
CSB-EP771832FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Glycerol kinase (glpK) CSB-YP771833FGB
CSB-EP771833FGB
CSB-BP771833FGB
CSB-MP771833FGB
CSB-EP771833FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Arginine deiminase (arcA) CSB-YP771834FGB
CSB-EP771834FGB
CSB-BP771834FGB
CSB-MP771834FGB
CSB-EP771834FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0213 protein PP_0972 (PP_0972) CSB-YP771835FGB
CSB-EP771835FGB
CSB-BP771835FGB
CSB-MP771835FGB
CSB-EP771835FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida UPF0307 protein PP_0941 (PP_0941) CSB-YP771836FGB
CSB-EP771836FGB
CSB-BP771836FGB
CSB-MP771836FGB
CSB-EP771836FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Superoxide dismutase [Fe] (sodB) CSB-YP771837FGB
CSB-EP771837FGB
CSB-BP771837FGB
CSB-MP771837FGB
CSB-EP771837FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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