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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Solanum lycopersicum Gamma aminobutyrate transaminase 2 (GABA-TP2) CSB-YP771154SZY
CSB-EP771154SZY
CSB-BP771154SZY
CSB-MP771154SZY
CSB-EP771154SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 17.9 kDa class I heat shock protein (HSP17.9A) CSB-YP771155OFG
CSB-EP771155OFG
CSB-BP771155OFG
CSB-MP771155OFG
CSB-EP771155OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Phosphoenolpyruvate/phosphate translocator 2, chloroplastic (PPT2), partial CSB-YP771156OFG1
CSB-EP771156OFG1
CSB-BP771156OFG1
CSB-MP771156OFG1
CSB-EP771156OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH53 (BHLH53) CSB-YP771157DOA
CSB-EP771157DOA
CSB-BP771157DOA
CSB-MP771157DOA
CSB-EP771157DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable E3 ubiquitin-protein ligase ARI15 (ARI15) CSB-YP771158DOA
CSB-EP771158DOA
CSB-BP771158DOA
CSB-MP771158DOA
CSB-EP771158DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Zinc finger CCCH domain-containing protein 50 (Os07g0568300, LOC_Os07g38090), partial CSB-YP771159OFG
CSB-EP771159OFG
CSB-BP771159OFG
CSB-MP771159OFG
CSB-EP771159OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein phosphatase 2C 35 (XB15), partial CSB-YP771160OFG
CSB-EP771160OFG
CSB-BP771160OFG
CSB-MP771160OFG
CSB-EP771160OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Monothiol glutaredoxin-S13 (GRXS13) CSB-YP771161DOA
CSB-EP771161DOA
CSB-BP771161DOA
CSB-MP771161DOA
CSB-EP771161DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein NLP7 (NLP7), partial CSB-YP771162DOA
CSB-EP771162DOA
CSB-MP771162DOA
CSB-EP771162DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutathione S-transferase F4 (GSTF4) CSB-YP771163DOA
CSB-EP771163DOA
CSB-BP771163DOA
CSB-MP771163DOA
CSB-EP771163DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Homeobox-leucine zipper protein HOX2 (HOX2) CSB-YP771164OFF
CSB-EP771164OFF
CSB-BP771164OFF
CSB-MP771164OFF
CSB-EP771164OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana S- (+)-linalool synthase, chloroplastic (TPS14), partial CSB-YP771165DOA
CSB-EP771165DOA
CSB-BP771165DOA
CSB-MP771165DOA
CSB-EP771165DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chenopodium album Profilin CSB-YP771166CIA
CSB-EP771166CIA
CSB-BP771166CIA
CSB-MP771166CIA
CSB-EP771166CIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At2g17525, mitochondrial (At2g17525), partial CSB-YP771167DOA
CSB-EP771167DOA
CSB-BP771167DOA
CSB-MP771167DOA
CSB-EP771167DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphoenolpyruvate carboxylase 3 (PPC3), partial CSB-YP771168DOA
CSB-EP771168DOA
CSB-BP771168DOA
CSB-MP771168DOA
CSB-EP771168DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein cornichon homolog 4 (At1g12390), partial CSB-YP771169DOA1
CSB-EP771169DOA1
CSB-BP771169DOA1
CSB-MP771169DOA1
CSB-EP771169DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable mannan synthase 1 (CSLA1), partial CSB-YP771170DOA1
CSB-EP771170DOA1
CSB-BP771170DOA1
CSB-MP771170DOA1
CSB-EP771170DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ubiquitin carboxyl-terminal hydrolase 7 (UBP7) CSB-YP771171DOA
CSB-EP771171DOA
CSB-BP771171DOA
CSB-MP771171DOA
CSB-EP771171DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Sodium/hydrogen exchanger 3 (NHX3), partial CSB-YP771172DOA
CSB-EP771172DOA
CSB-BP771172DOA
CSB-MP771172DOA
CSB-EP771172DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein REM17 (REM17), partial CSB-YP771173DOA
CSB-EP771173DOA
CSB-BP771173DOA
CSB-MP771173DOA
CSB-EP771173DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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