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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coxiella burnetii UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP772652DXP
CSB-EP772652DXP
CSB-BP772652DXP
CSB-MP772652DXP
CSB-EP772652DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP772653DXP
CSB-EP772653DXP
CSB-BP772653DXP
CSB-MP772653DXP
CSB-EP772653DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Protein TolB (tolB) CSB-YP772654DXP
CSB-EP772654DXP
CSB-BP772654DXP
CSB-MP772654DXP
CSB-EP772654DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Uncharacterized protein CBU_0041 (CBU_0041), partial CSB-YP772655DXP
CSB-EP772655DXP
CSB-BP772655DXP
CSB-MP772655DXP
CSB-EP772655DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA replication and repair protein recF (recF) CSB-YP772656DXP
CSB-EP772656DXP
CSB-BP772656DXP
CSB-MP772656DXP
CSB-EP772656DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Orotate phosphoribosyltransferase (pyrE) CSB-YP772657TIW
CSB-EP772657TIW
CSB-BP772657TIW
CSB-MP772657TIW
CSB-EP772657TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L7/L12 (rplL) CSB-YP772658TIW
CSB-EP772658TIW
CSB-BP772658TIW
CSB-MP772658TIW
CSB-EP772658TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 30S ribosomal protein S12 (rpsL) CSB-YP772659TIW
CSB-EP772659TIW
CSB-BP772659TIW
CSB-MP772659TIW
CSB-EP772659TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP772660TIW
CSB-EP772660TIW
CSB-BP772660TIW
CSB-MP772660TIW
CSB-EP772660TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 30S ribosomal protein S10 (rpsJ) CSB-YP772661TIW
CSB-EP772661TIW
CSB-BP772661TIW
CSB-MP772661TIW
CSB-EP772661TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L22 (rplV) CSB-YP772662TIW
CSB-EP772662TIW
CSB-BP772662TIW
CSB-MP772662TIW
CSB-EP772662TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 50S ribosomal protein L5 (rplE) CSB-YP772663TIW
CSB-EP772663TIW
CSB-BP772663TIW
CSB-MP772663TIW
CSB-EP772663TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Translation initiation factor IF-1 (infA) CSB-YP772664TIW
CSB-EP772664TIW
CSB-BP772664TIW
CSB-MP772664TIW
CSB-EP772664TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP772665TIW
CSB-EP772665TIW
CSB-BP772665TIW
CSB-MP772665TIW
CSB-EP772665TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei ATP-dependent Clp protease proteolytic subunit 1 (clpP1) CSB-YP772666TIW
CSB-EP772666TIW
CSB-BP772666TIW
CSB-MP772666TIW
CSB-EP772666TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Octanoyltransferase (lipB) CSB-YP772667TIW
CSB-EP772667TIW
CSB-BP772667TIW
CSB-MP772667TIW
CSB-EP772667TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Ribosome-recycling factor (frr) CSB-YP772668TIW
CSB-EP772668TIW
CSB-BP772668TIW
CSB-MP772668TIW
CSB-EP772668TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei DNA ligase (ligA), partial CSB-YP772669TIW
CSB-EP772669TIW
CSB-BP772669TIW
CSB-MP772669TIW
CSB-EP772669TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP772670TIW
CSB-EP772670TIW
CSB-BP772670TIW
CSB-MP772670TIW
CSB-EP772670TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei 3-dehydroquinate synthase (aroB) CSB-YP772671TIW
CSB-EP772671TIW
CSB-BP772671TIW
CSB-MP772671TIW
CSB-EP772671TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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