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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Ribose-5-phosphate isomerase A (rpiA) CSB-YP772252BAM
CSB-EP772252BAM
CSB-BP772252BAM
CSB-MP772252BAM
CSB-EP772252BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative UPF0397 protein BC_2624 (BC_2624), partial CSB-YP772253BAM1
CSB-EP772253BAM1
CSB-BP772253BAM1
CSB-MP772253BAM1
CSB-EP772253BAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP772254BAM
CSB-EP772254BAM
CSB-BP772254BAM
CSB-MP772254BAM
CSB-EP772254BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Chromosome-anchoring protein racA (racA) CSB-YP772255BAM
CSB-EP772255BAM
CSB-BP772255BAM
CSB-MP772255BAM
CSB-EP772255BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP772256BAM
CSB-EP772256BAM
CSB-BP772256BAM
CSB-MP772256BAM
CSB-EP772256BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Deoxyribose-phosphate aldolase (deoC) CSB-YP772257BAM
CSB-EP772257BAM
CSB-BP772257BAM
CSB-MP772257BAM
CSB-EP772257BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable D-serine dehydratase (dsdA) CSB-YP772258BAM
CSB-EP772258BAM
CSB-BP772258BAM
CSB-MP772258BAM
CSB-EP772258BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartate 1-decarboxylase (panD) CSB-YP772259BAM
CSB-EP772259BAM
CSB-BP772259BAM
CSB-MP772259BAM
CSB-EP772259BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-dehydroquinate synthase (aroB) CSB-YP772260BAM
CSB-EP772260BAM
CSB-BP772260BAM
CSB-MP772260BAM
CSB-EP772260BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP772261BAM
CSB-EP772261BAM
CSB-BP772261BAM
CSB-MP772261BAM
CSB-EP772261BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP772262BAM
CSB-EP772262BAM
CSB-BP772262BAM
CSB-MP772262BAM
CSB-EP772262BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Histidinol dehydrogenase (hisD) CSB-YP772263BAM
CSB-EP772263BAM
CSB-BP772263BAM
CSB-MP772263BAM
CSB-EP772263BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0180 protein BC_1394 (BC_1394) CSB-YP772264BAM
CSB-EP772264BAM
CSB-BP772264BAM
CSB-MP772264BAM
CSB-EP772264BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 7-carboxy-7-deazaguanine synthase (queE) CSB-YP772265BAM
CSB-EP772265BAM
CSB-BP772265BAM
CSB-MP772265BAM
CSB-EP772265BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Tryptophan synthase alpha chain (trpA) CSB-YP772266BAM
CSB-EP772266BAM
CSB-BP772266BAM
CSB-MP772266BAM
CSB-EP772266BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cardiolipin synthase 2 (cls2), partial CSB-YP772267BAM1
CSB-EP772267BAM1
CSB-BP772267BAM1
CSB-MP772267BAM1
CSB-EP772267BAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0234 protein BC_1159 (BC_1159) CSB-YP772268BAM
CSB-EP772268BAM
CSB-BP772268BAM
CSB-MP772268BAM
CSB-EP772268BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Major cold shock protein CspA (cspA) CSB-YP772269BAM
CSB-EP772269BAM
CSB-BP772269BAM
CSB-MP772269BAM
CSB-EP772269BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0145 protein BC_1012 (BC_1012) CSB-YP772270BAM
CSB-EP772270BAM
CSB-BP772270BAM
CSB-MP772270BAM
CSB-EP772270BAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable disulfide formation protein C (bdbC), partial CSB-YP772271BAM1
CSB-EP772271BAM1
CSB-BP772271BAM1
CSB-MP772271BAM1
CSB-EP772271BAM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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