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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant UPF0229 protein VP0986 (VP0986) CSB-YP771638VFE
CSB-EP771638VFE
CSB-BP771638VFE
CSB-MP771638VFE
CSB-EP771638VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deoxyguanosinetriphosphate triphosphohydrolase-like protein (VP0925) CSB-YP771639VFE
CSB-EP771639VFE
CSB-BP771639VFE
CSB-MP771639VFE
CSB-EP771639VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable septum site-determining protein MinC (minC) CSB-YP771640VFE
CSB-EP771640VFE
CSB-BP771640VFE
CSB-MP771640VFE
CSB-EP771640VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA ligase (ligA), partial CSB-YP771641VFE
CSB-EP771641VFE
CSB-BP771641VFE
CSB-MP771641VFE
CSB-EP771641VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP771642VFE
CSB-EP771642VFE
CSB-BP771642VFE
CSB-MP771642VFE
CSB-EP771642VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable rRNA maturation factor (VP0731) CSB-YP771643VFE
CSB-EP771643VFE
CSB-BP771643VFE
CSB-MP771643VFE
CSB-EP771643VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lipoyl synthase (lipA) CSB-YP771644VFE
CSB-EP771644VFE
CSB-BP771644VFE
CSB-MP771644VFE
CSB-EP771644VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP771645VFE
CSB-EP771645VFE
CSB-BP771645VFE
CSB-MP771645VFE
CSB-EP771645VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gamma-glutamyl phosphate reductase (proA) CSB-YP771646VFE
CSB-EP771646VFE
CSB-BP771646VFE
CSB-MP771646VFE
CSB-EP771646VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP771647VFE
CSB-EP771647VFE
CSB-BP771647VFE
CSB-MP771647VFE
CSB-EP771647VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP771648VFE
CSB-EP771648VFE
CSB-BP771648VFE
CSB-MP771648VFE
CSB-EP771648VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptidase B (pepB) CSB-YP771649VFE
CSB-EP771649VFE
CSB-BP771649VFE
CSB-MP771649VFE
CSB-EP771649VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP771650VFE
CSB-EP771650VFE
CSB-BP771650VFE
CSB-MP771650VFE
CSB-EP771650VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP771651VFE
CSB-EP771651VFE
CSB-BP771651VFE
CSB-MP771651VFE
CSB-EP771651VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S20 (rpsT) CSB-YP771652VFE
CSB-EP771652VFE
CSB-BP771652VFE
CSB-MP771652VFE
CSB-EP771652VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysine--tRNA ligase (lysS), partial CSB-YP771653VFE
CSB-EP771653VFE
CSB-BP771653VFE
CSB-MP771653VFE
CSB-EP771653VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP771654VFE
CSB-EP771654VFE
CSB-BP771654VFE
CSB-MP771654VFE
CSB-EP771654VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP771655VFE
CSB-EP771655VFE
CSB-BP771655VFE
CSB-MP771655VFE
CSB-EP771655VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP771656VFE
CSB-EP771656VFE
CSB-BP771656VFE
CSB-MP771656VFE
CSB-EP771656VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase (uppP), partial CSB-YP771657VFE
CSB-EP771657VFE
CSB-BP771657VFE
CSB-MP771657VFE
CSB-EP771657VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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