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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Coxiella burnetii 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP772612DXP
CSB-EP772612DXP
CSB-BP772612DXP
CSB-MP772612DXP
CSB-EP772612DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP-dependent protease subunit HslV (hslV) CSB-YP772613DXP
CSB-EP772613DXP
CSB-BP772613DXP
CSB-MP772613DXP
CSB-EP772613DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Acylphosphatase (acyP) CSB-YP772614DXP
CSB-EP772614DXP
CSB-BP772614DXP
CSB-MP772614DXP
CSB-EP772614DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP synthase subunit beta (atpD) CSB-YP772615DXP
CSB-EP772615DXP
CSB-BP772615DXP
CSB-MP772615DXP
CSB-EP772615DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP synthase subunit a (atpB), partial CSB-YP772616DXP1
CSB-EP772616DXP1
CSB-BP772616DXP1
CSB-MP772616DXP1
CSB-EP772616DXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Peptide deformylase 2 (def2) CSB-YP772617DXP
CSB-EP772617DXP
CSB-BP772617DXP
CSB-MP772617DXP
CSB-EP772617DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Outer-membrane lipoprotein LolB (lolB) CSB-YP772618DXP
CSB-EP772618DXP
CSB-BP772618DXP
CSB-MP772618DXP
CSB-EP772618DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phosphoglycerate kinase (pgk) CSB-YP772619DXP
CSB-EP772619DXP
CSB-BP772619DXP
CSB-MP772619DXP
CSB-EP772619DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Phosphoheptose isomerase (gmhA) CSB-YP772620DXP
CSB-EP772620DXP
CSB-BP772620DXP
CSB-MP772620DXP
CSB-EP772620DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP772621DXP
CSB-EP772621DXP
CSB-BP772621DXP
CSB-MP772621DXP
CSB-EP772621DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosomal protein S6 modification protein (rimK) CSB-YP772622DXP
CSB-EP772622DXP
CSB-BP772622DXP
CSB-MP772622DXP
CSB-EP772622DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP772623DXP
CSB-EP772623DXP
CSB-BP772623DXP
CSB-MP772623DXP
CSB-EP772623DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Protein-export protein SecB (secB) CSB-YP772624DXP
CSB-EP772624DXP
CSB-BP772624DXP
CSB-MP772624DXP
CSB-EP772624DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Glutamate--tRNA ligase 2 (gltX2) CSB-YP772625DXP
CSB-EP772625DXP
CSB-BP772625DXP
CSB-MP772625DXP
CSB-EP772625DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP772626DXP
CSB-EP772626DXP
CSB-BP772626DXP
CSB-MP772626DXP
CSB-EP772626DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosome-binding factor A (rbfA) CSB-YP772627DXP
CSB-EP772627DXP
CSB-BP772627DXP
CSB-MP772627DXP
CSB-EP772627DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosome-recycling factor (frr) CSB-YP772628DXP
CSB-EP772628DXP
CSB-BP772628DXP
CSB-MP772628DXP
CSB-EP772628DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii D-alanine--D-alanine ligase (ddl) CSB-YP772629DXP
CSB-EP772629DXP
CSB-BP772629DXP
CSB-MP772629DXP
CSB-EP772629DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii SsrA-binding protein (smpB) CSB-YP772630DXP
CSB-EP772630DXP
CSB-BP772630DXP
CSB-MP772630DXP
CSB-EP772630DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Lipoyl synthase (lipA) CSB-YP772631DXP
CSB-EP772631DXP
CSB-BP772631DXP
CSB-MP772631DXP
CSB-EP772631DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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